Expressions And Significance Of YAP/TAZ In Human Breast Cancer

Objective:The expression of TAZ,YAP,CD44,and CD24 in breast cancer patients was observed via immunohistochemistry.And use statistical methods to compare and analyze the general clinical data of patients.To investigate the relationship between Hippo signaling pathway and cancer stem cells and cancer invasion and metastasis in human breast cancer,and to provide theoretical basis for the clinical development of novel therapeutic methods for breast cancer.Methods:From December 2016 to January 2018,32 postoperative paraffin-embedded specimens of breast cancer patients undergoing concurrent radical mastectomy,complete clinical and pathological data,were collected.Simultaneously collecting normal breast tissue(>2 cm from the edge of tumor tissue,no tumor cells stained with HE).To observe the expression of TAZ,YAP,CD44,and CD24 in breast cancer patients via immunohistochemistry.Chi-square test was used to analyze the differences in protein expression between breast cancer tissues and adjacent tissues.Spearman’s correlation coefficient was used to analyze the correlation between protein expression and clinical information of patients.Results:1.The positive expression rate of AP was 78.13%(25/32).The positive expression rate of YAP in adjacent tissues was 19.05%(4/21),c2 = 17.80,p= 0.000<0.05,the difference was statistically significant;2.The positive expression rate of TAZ was 59.38%(19/32),and the positive expression rate of TAZ in adjacent tissues was 14.29%(3/21),c2 = 10.617,p= 0.001<0.05,the difference was statistically significant;3.The positive expression rate of CD44 was 53.13%(17/32).The positiveexpression rate of CD44 in the adjacent tissues was 9.52%(2/21),c2 = 10.481,p= 0.001 <0.05,the difference was statistically significant;4.The positive expression rate of CD24 was 65.63%(21/32),and the positiveexpression rate of CD24 in adjacent tissues was 28.57%(6/21),c2 = 6.966,p = 0.008<0.05,the difference was statistically significant;5.The positive expression rate of MMP9 was 56.25%(18/32),and the positiveexpression rate of MMP9 was found in the adjacent tissues.The difference was statistically significant(23.81%,5/21).The comparison was c 2 =5.432,p=0.02<0.05;6.The positive expression rate of MMP2 was 50.00%(16/32),and the positive expression rate of MMP2 was found in the adjacent tissues.The difference was statistically significant(23.81%,4/21).c2 = 5.17,p= 0.023 <0.05.7.There was a significant positive correlation between TAZ protein expressionand YAP protein expression in breast cancer tissues(2=7.552,p=0.006<0.05;r=0.486,p=0.005<0.05);There was a significant positive correlation with CD44 protein expression(2=4.394,p=0.036<0.05;r=0.371,p=0.037<0.05);and MMP9 egg The expression was significantly positively correlated(2=5.776,p=0.016<0.05;r=0.425,p=0.015<0.05);there was also a significant positive correlation with MMP2 protein expression(2=6.348,p=0.011<0.05;r=0.445,p=0.011<0.05);8.In breast cancer tissues,there was a significant positive correlation between YAP protein expression and TAZ protein expression(2=7.552,p=0.006<0.05;r=0.486,p=0.005<0.05);There was a significant positive correlation with CD44 protein expression(2=5.428,p=0.02<0.05;r=0.412,p=0.019<0.05);and CD24 protein The expression was significantly positively correlated(2=5.453,p=0.02<0.05;r=0.413,p=0.019<0.05);9.Positive expression of TAZ protein and invasive breast cancer(2=11.792,p=0.001<0.05;r=0.607,p=0.000<0.05),distant metastasis(2=4.394,p=0.036<0.05;r=0.371,p=0.037<0.05)were statistically significant and all showed significant positive correlation;10.YAP protein expression and invasive breast cancer(2=6.732,p=0.009<0.05;r=0.459,p=0.008<0.05),distant metastasis(2=6.412,p=0.011<0.05;r=0.448,p=0.01<0.05).There was a statistically significant difference and there was a significant positive correlation.Conclusion:1.In breast cancer tissues,the expression of CD44,CD24,YAP,TAZ,MMP9 and MMP2 proteins was higher than that in the adjacent tissues;2.Hippo signaling pathway plays an important role in the development of breast cancer,which may be achieved through the regulation of CD44/CD24 and MMPs protein expression.