Fabrication Of UV-assited Treatment On Intraocular Lens Anterior Surface With 2-methacryloyloxyethl Phosphorylcholine And Biological Detection In Vitro

Objective:To evaluate the feasibility of UV-assisted treatment on intraocular lens anterior(IOL)surface with 2-methacryloyloxyethyl phosphorylcholine(MPC).Design a new IOL of Hydrophilic anterior surface and Hydrophobic posterior surface.To evaluate the efficacy and safety of new IOL of reducing postoperative inflammatory reaction and inhibiting PCO develooment.Methods:MPC was grafted onto the anterior surface of hydrophobic acrylic intraocular lens by UV curing process.X-ray photoelectron spectroscopy(XPS)and Water contact angle(WCA)was used to test the surface chemical element changes and hydrophilicity of new IOL;Field emission scanning electron microscopy(FESEM)and atomic force microscopy(AFM)were used to observe the surface morphological.According to IOL National Standard,the optical and mechanical properties were tested on the new IOL.The methods of cell adhesion assay and AO/PI double staining were used to analyze the cell density and morphological changes,the survival and apoptosis of adhesion cells on the new IOL surface;Detect the expression of adhesion and differentiation proteins in LECs on the new IOL surface.Observe the migrations of LECs between the new IOL and the simulate posterior capsule in simulate human capsule environment.Results:After analysis,the new IOL surface had been successfully grafted with MPC,the surface hydrophilicity enhanced and tended to be stable afer 180 seconds;The CFDA report revealed that the new IOL was in line with the IOL National Standard.Compared with the control group,the new IOL had no difference on the surface morphology,butthe surface roughness was reduced.Biological examination in vitro revealed,the adhesion of macrophages and LECs on the anterior surface of the new IOL was significantly lower than that of the control group(P<0.005,Wilcoxon test),and the cells were not activated.AO/PI double staining results revealed that the LEC cells survival status on the surface of new IOL was higher than control group;The immunofluorescence results showed that there were no significantly difference in the protein expression of Laminin,ICAM-1,Fibronectin and Alpha-SMA between the two groups.The successful establishment of in vitro simulated human capsule model,therewas no difference between the new IOL and the control group of crawling resules,then the posterior surface treatment group was statistically significant than former two groups.Conclusions:The new hydrophobic acrylic IOL was successfully fabricated by UV curing technology assisted MPC grafted onto the front surface of IOL.The new IOL could enhance the hydrophilicity of the front surface,effectively reduce the adhesion of the inflammatory cells,while maintaining the hydrophobicity of the posterior surface to inhibit the development of PCO.