The Research Of MD Inhibits The Expression Of Apo(a) And Explore Its Primarily Mechanism

Object:To study the expression of apolipoprotein(a)[apo(a)] in Hep G2 cells treated by Momordicin[MD] and explore its mechanism.Method: 1.Using centrifugence,dialysis,superfitrate,cation-exchange chromatography and reverse-Phase high performance liquid choromatography to purify the protein from momordical;2.Hep G2 cell line,an high apo(a)expression cell,was used for this experiments;3.MTT assay was used to observe the survival ratio of Hep G2 cell treated with different concentrations of MD(0,0.1,0.2,0.4,0.8,1.6,3.2mg/ml)for 24 h or treated with 0.8mg/ml MD fordifferent time(0,6,12,24,48h);4.Western blot was used to detect the expression of apo(a),FXR and HNF4α in Hep G2 cells treated with different concentrations of MD(0,0.1,0.2,0.4,0.8,1.6mg/ml)for 24 h or treated with 0.8mg/ml MD fordifferent time(0,6,12,24,48h);5.Quantitative Real-time PCR was used to detect the expression of apo(a),FXR and HNF4α m RNA level in Hep G2 cells treated with different concentrations MD(0,0.1,0.2,0.4,0.8,1.6mg/ml)for 24 h or treated with 0.8mg/ml MD for different time(0,6,12,24,48h).Result:1.The MTT results showed that compared with control group,there were no significant difference for the livability of Hep G2 cells treated with MD(0,0.1,0.2,0.4,0.8,1.6mg/ml)for 24 h,while the livability of Hep G2 cells treated with 3.2mg/ml MD decreased compared with control group;the livability of Hep G2 cells between control group and MD group was not significant for different time(0,6,12,24,48 h);2.Western blot and RT-PCR results showed that compared with control group,MD dose and time dependently increased FXR,reduced apo(a)and HNF4α expression in protein and m RNA level.Conclusion:1.MD inhibits apo(a)expression;2.The mechanism of MD inhibits apo(a)expression may be related to FXR/HNF4α.