| Elucidation of chemical composition of Chinese patent medicine plays an important role in its redevelopment process since it is not only helpful for the improvement of quality control standard but also is the primary foundation of understanding in vivo metabolisms and mechanisms of action of Chinese patent medicine."FU-KE-ZAI_ZAO" pill(FKW)is composed of 42 Chinese medicinal herbs,which has been widely used to treat various gynecological diseases.However,due to its chemical complexity,there is no study available on chemical basis of FKW,which significantly limits its clinical applications and in-depth development.Here FKW is selected as the research subject and liquid Chromatography-Mass Spectrometry is employed to perform a systemic study on chemical basis of FKW.The main results are summarized as following:1.A LC-MS method was successfully established to reveal the chemical substance in"FU-KE-ZAI-ZAO" pill.In this study,a total of 83 compounds(including Benzoic acid,flavonoids,terpenoids,triterpene glycosides and phthalides)were detected and tentatively identified,and most of these compounds were assigned.2.A LC-MS method was constructed to analysis the prototype compounds and their metabolites of rat plasma samples after administration of "FU-KE-ZAI-ZAO" pill.As a result,21 prototype compounds and 5 metabolites were detected and tentatively identified or tentatively elucidated their structures.From this study,the blood IV component of FKW was determined preliminarily and could be useful to the further study.3.A LC-MS fingerprint including 44 common characteristic peaks were developed by selected ions monitoring(SIM)mode,and the method was applied to the evaluation of the batch consistency of 19 batches of FKW samples.The result showed that the consistency between the 19 batches was good,but the preparations of different years still had some differences. |
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