Study On The Differential Expression Profiles Of MiRNAs In Plasma Of Patients With Systemic Lupus Erythematosus

Objective: The Differential expression profiles of miRNAs of plasma of patients with Systemic lupus erythematosus(SLE)and healthy persons were obtained by miRNA chip technology.In order to obtain the biomarkers for diagnosis and treatment of SLE.Methods: Qiagen reagent was used to obtained total RNA from the plasma of 18 patients with SLE and 10 healthy individuals.mi RNA chip was used to detect the differential expression of miRNAs in plasma of patients with SLE and healthy people.The expression level of two miRNAs with the largest difference were detected by qRT-PCR.And compare to the result of gene chip.To see the role of the two miRNAs in occurence and development of SLE.Results:1.The differentially expression profiles of miRNAs of patients with SLE were obtained.In SLE patients,the number of significant ly upregulated miRNAs were 35,mi R-21 has the biggest times of upregulation with 26.1235 times.The number of significantly downregulated miRNAs were 14.mi R-146 a has the biggest times of dow nregulation with 0.0512 times.2.In 18 patients with SLE,the relative mean values of miR-21 were33.5±2.2 and 2.8±0.5 in 10 cases of healthy individuals,the upregulated times were 11.9642.In 18 cases of SLE patients,the relative mean values of miR-146 a were 0.3±0.1 and 5.7±1.3 in 10 cases of healthy individuals,the downregulated times were 0.2281.3.The results of mi R-21 and miR-146 a in qRT-PCR were consistent with those of gene chip,which indicated that the result of gene chip were reliable.4.Bioinformatics analysis showed that the numbe of target genes of miR-21 were 307 and that involved signaling pathways including EGF,TGF-beta,MAPK,Jak-STAT and neurotrophic factor signaling pathways;the numbe of target genes of mi R-146 a were 224 and that involved signaling pathways including MAPK,Notch and Fas signaling pathways.Conclusion:1.The mi RNAs differential expression profiles were obtained.In the SLE plasma,the number of significantly upregulated mi RNAs were 35,and the number of significantly downregulated mi RNAs were 14.2.In the SLE patients,miR-21 has the biggest times of upregulation and mi R-146 a has the biggest times of downregulation.The results of RT-PCR of two mi RNAs was consistent with that of gene chip.3.miR-146 a and mi R-21 may play an important roles in the occurrence and development of SLE.