Analysis For The Effect Of Laser-assisted Hatching On Pregnancy Outcomes In The Women With Polycystic Ovary Syndrome Undergoing In Vitro Fertilization-embryo Transfer

Objective:To explore the effect of laser-assisted hatching on pregnancy outcomes in the women with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer.Methods:A prospective study was conducted on the women with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer in our reproductive center from January,2012 to December,2014.A total of 163 patients were included in the study and divided into two groups:groupⅠ(age<35 or BMI<23kg/m~2);groupⅡ(age≥35 or BMI≥23kg/m~2).In each group embryos were cultured and transferred with or without laser-assisted hatching(LAH or NLAH).Separately compare the basic clinical feathers,oocyte and embryo conditions,and pregnancy outcome between the two groups.Results:(1)When patients aged<35years,clinical pregnancy rates and embryo implantation rates were not significantly different between group LAH(n=29)and group NLAH(n=33)(P>0.05)。(2)When patients aged≥35 years,the embryo implantation and multiple pregnancy rates in the group LAH(n=55)were significantly higher than those in the group NLAH(n=46)(29.33%vs 19.08%and46.67%vs 8.70%respectively).(P<0.05).(3)In group I(BMI<23kg/m~2),clinical pregnancy rates and embryo implantation rates were not significantly different between group LAH(n=38)and group NLAH(n=38)(P>0.05)。(4)In group II(BMI≥23kg/m~2),clinical pregnancy rates and embryo implantation rates were not significantly different between group LAH(n=46)and group NLAH(n=41)(P>0.05)。Conclusions:(1)Laser-assisted hatching can improve the embryo implantation rate in women aged≥35years with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer.Laser-assisted hatching can not improve pregnancy outcomes in women aged<35 years with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer.(2)Laser-assisted hatching could result in an increasing incidence of multiple pregnancy in women aged≥35 years with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer.Thus,the application of laser-assisted hatching in clinical was still of limitation.(3)Laser-assisted hatching can not improve pregnancy outcomes in normal weight or obese women with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer.