Study On The Vascular Mechanism Of Treating The Scleroderma By Dangguisini Decoction On AECA,vWF,TXB₂,6-keto-PGF1_? From Scleroderma Model Mice

Objective:The study is to evaluate the curative effect and dose-effect relationship for scleroderma mice,discusse possible mechanism of action for scleroderma of Dangguisini decoction,and provide the basis for clinic treatment of systemic sclerosis by means of observing each group,to study the effect on content of anti-endothelial cell antibody?AECA?in circular blood serum,von Willebrand factor?vWF?,thromboxane B₂?TXB₂?and 6-keto-ProstaglandinFla?6-keto-PGF1??in adtevak in scleroderma mice,and the pathological alteration of lung and skin.Methods:1 Group the experiment:randomly divide the ninety female BALB/c mice into six groups:high-dose,middle-dose,low-dose of Dangguisini decoction,Bozhi tablets,model set and blank group,with each group of fifteen mice.Adaptively feed the mice for 6 days,and then unhair the back of mice three days before the experiment.2 Establish the Animal Model:give hypodermic injection of0.1ml Phosphate Buffered Saline into the hair removal space of the blank group mice,the rest group 0.1ml of200ug/ml concentration Bleomycin,one time one day for 21 days.3 Pharmacological intervention:after the success of animal modeling,high-dose,middle-dose,lose-dose of Dangguisini decoction respectively use intragastric administration of high,medium and low concentration of Dangguisini water decoctum,Bozhi tablets using Bozhi solution,model set and blank group using normal saline for 21 days.4 Collect specimen and test:sample the eyeball blood and separate serum from plasma.Using enzyme linked immuno sorbent assay?ELISA?to test the content of TXB2,6-keto-PGF1?and vWF in plasma and AECA in serum.Fetch mice lungs and areas of skin tissue fixing with 10%formaldehyde solution,and then with paraffin embedding,section and HE dyeing.Observe pathological changes of lung and skin under the microscope.Results:1 The modeling of Scleroderma BALB/c female mice induced by BLM is successful.2 Pathological changes of lung and skin:With the pachulosis,hardness enhancement,incrassation of corium layer,thickening and densement of collagenous fiber,hyperplasia of prickle cell layer,reduce of blood vessel underlying dermis and inflammatory cell infiltration in model set,Dangguisini decoction groups changed lightly.With incrassation of lung vascular wall and thin alveolar wall,increase of collagenous fiber,hyperplasia of mesenchymal cell and lymphocytes infiltration in model set,Dangguisini decoction groups changed lightly.3 Content changes of vWF,AECA,TXB₂,6-Keto-PGF1?in plasma or serum:?1?As for the concentration of AECA in serum,model set is higher than the blank group apparently.The difference has a statistical significance?P<0.01?;Bozhi tablets set is lower than model set.The difference has a statistical significance?P<0.01?;Dangguisini decoction groups are all lower than the model set,which has a statistical significance?P<0.01?;Dangguisini decoction groups are all higher than Bozhi tablets set,which has a statistical significance?P<0.01?;the concentration of AECA decrease progressively along with the increase of the Dangguisini decoction concentration.?2?As for the concentration of vWF in serum,model set is higher than the blank group apparently.The difference has a statistical significance?P<0.01?;Bozhi tablets set is lower than the model set.The difference has a statistical significance?P<0.01?;Dangguisini decoction groups are all lower than the model set,which has a statistical significance?P<0.01?;Dangguisini decoction groups are all higher than Bozhi tablets set,which has a statistical significance?P<0.01?;the concentration of vWF decrease progressively along with the increase of the Dangguisini decoction concentration.?3?As for the concentration of TXB₂ in serum,model set is higher than the blank group apparently.The difference has a statistical significance?P<0.01?;Bozhi tablets set is lower than the model set.The difference has a statistical significance?P<0.01?;Dangguisini decoction groups are all lower than the model set,which has a statistical significance?P<0.01?;middle-dose and low-dose of Dangguisini decoction groups are all higher than Bozhi tablets set.The difference has a statistical significance?P<0.01?;high-dose of Dangguisini decoction group is higher than Bozhi tablets set,which doesn't have a statistical significance?P>0.05?;the concentration of TXB₂ decrease progressively along with the increase of the Dangguisini decoction concentration.?4?As for the concentration of 6-keto-PGF1?in serum,model set is lower than the blank group apparently.The difference has a statistical significance?P<0.01?;Bozhi tablets set is higher than the model set.The difference has a statistical significance?P<0.01?;Dangguisini decoction groups are all higher than the model set,which has a statistical significance?P<0.01?;Dangguisini decoction groups are all lower than Bozhi tablets set,which has a statistical significance?P<0.01?;the concentration of 6-keto-PGF1?increase progressively along with the increase of the Dangguisini decoction concentration.Conclusion:1 The increase of TXB₂,vWF,AECA concentration and the decrease of 6-Keto-PGF1?concentration which is related to the morbidity of SSc can be as the index to test condition and evaluate the therapeutic effect.2 Dangguisini decoction has a good effect on scleroderma mice,which is gradually strengthened along with the increase of the medicine concentration.This exists an obvious dose-effect relationship.3 The action mechanism of treating SSc mice with Dangguisini decoction could be achieved by decreasing the concentration of TXB₂,vWF,AECA and increasing the6-Keto-PGF1?concentration in mice blood.4 The therapeutic action on SSc mice with Dangguisini decoction could be achieved by adjusting Yingwei system function.