A Study About Bu Yang Huan Wu Tang And Demolitioh Party Influence On The Blood Brain Barrier Model Of Permeability (Liver-kidney Yin Deficiency Type)

Objective:Survey the effect of BuYang Huan Wu Tang and demolitioh party on the Blood Brain Barrier Model of permeabilith.Probe into the method of treat ischemic stroke.Understanding different inscape of effect value.Offering laboratory suustain for clinical component.Methods:The article extract the BCECs(Brain Capillary Endothelial Cells)and Astrocytes from rats brain.Establishing the BBBM(Blood Brain Barrier Model)needs porous bottom plate and 12well culture plates use cooperatively,andits basement is the polyester film more.On which are 3um dia spread all over,and it is coated by 0.01%rat tail collagen,When astrocyte of second generation mix together to80%,cell dissociation,centrifugation,cell counting,and situate below the polyester film more,cell density is 5×10~4?/cm~2,200ul/hole,and join the DMEM culture medium and bFGFlng/ml.After 24h,when BCECs of second generation mix together to 80%,cell dissociation,centrifugation,cell counting,and vaccination the BCECs above the polyester film more,cell density is 5×10~4?/cm~2,200ul/hole.Medium is same to Aatrocyte.48 hours later,use the microscope observe the cell's growth and fusion situation.At the same time,the external patterns of BBB will be divided into the normal guoup,total party group,promoting blood circulation group and qi invigorating group,having 3 after hole each group.Every group inject drug serum respectively.By means of HRP(horsenadish peroxidase HRP)experiments,liquid interview leakage experiments,TEER(transendothelial electricalresistance TEER)experiments to judge the external patterns of BBB if or not form.The data process by the computer spass19.0 statistical analysis software.Expressd by data(x±s),comparison between groups by one-way ANOVA,T test can be used for normal distribution,the heterogeneity of variance corrected t test or paired t test,measurement data using x2test.To test the water by 0.05 quasi p<0.05 was statistically significant.Results:Compare to the normal group,the TEER of total party group,promoting blood circulation group and qi invigorating group take place with chang at the time(P<0.05).Two groups of patients before treatment ALS functional classification scale(ALSFRS)and TCM symptom integral by the statistical analysis has no obvious difference(P>0.05).Conclusions:Comprare normal guoup with total party group,promoting blood circulation group and qi invigorating group at the same time,HRP increase at a certain time(P<0.05).Comprare promoting blood circulation group and qi invigorating group at the same time,HRP of total party group grow signally(P<0.05).