Screening Of MiRNAs With Differentially Expressed Genes In Gastric Cancer And Its Biological Significance

Objective : Gene chip is widely used as a biochip currently,the research of gene chip can be displayed high-throughput in miRNA,which has a greater advantage to the molecular mechanism of the pathogenesis of gastric cancer.In this study,the miRNA gene data GSE63121 of human gastric cancer is analyzed based on bioinformatics method,with the different miRNAs screened out and its biological functions analyzed,and further to explore the molecular mechanism of gastric cancer.Method: Firstly,the miRNA gene microarray of human gastric cancer was retrieved from the GEO database,then GeneSpring GX 11.5software was used to analyze the gene microarray data through using paired T test statistical method.Following,the differentially expressed miRNAs screened were verified by RT-PCR.Secondly,the target genes of miRNAs were predicted through TargetScan,miRDB and PicTar online tools,by using DAVID software to analyze the gene ontology and signal pathway enrichment of target genes.Finally,combined with the relevant literature,these genes and the biological significance in signalingpathways in gastric cancer were analyzed.Results: A total of 22 different miRNAs were obtained by screening the GSE63121 gene of gastric cancer gene chip,including 18down-regulated miRNAs and 4 up-regulated mi RNAs(Differences are all more than 1.5 times).The RT-PCR validation results of mir-362-3p,mir-486-5p and mir-31 are consistent with the testing results of gene chip,which proved the reliability of experimental data.22 different miRNAs were introduced into TargetScan,miRDB and PicTar online software to predict target gene respectively,and 196 target genes were obtained by intersection.The results of gene ontology analysis showed that the biological processes include transcriptional regulation,RNA polymerase II transcriptional regulation,negative regulation of cell growth,transcription,regulation of circadian rhythm and sequence-specific DNA-binding transcription factor activity.The results of signal pathway enrichment analysis mainly includes HIF-1 signaling pathway,oxytocin signaling pathway,oocyte meiosis signaling pathway,AMPK signaling pathway,axon guidance,MAPK signaling pathway,Wnt signaling pathway and myocardial adrenergic signal.Conclusions: 1.We screened for differentially expressed mi RNAs by the gene chip data,and through analysis of biological function,we can find gastric cancer is related to miRNAs.2.The pathogenesis of gastric cancer may be associated with HIF-1signaling pathway,Wnt signaling pathway,MAPK signaling pathway and AMPK signaling pathway.3.MiR-31,miR-222,miR-27 b and miR-150,which are differentially expressed in gastric cancer,may be associated with the pathogenesis of gastric cancer.4.HIF-1 signaling pathway may be associated with drug resistance in chemotherapy by affecting CDKN1B.