Differentially Expressed Profiles Of LncRNA In Pterygium

Objective:The differential expression of lnc RNA in pterygium tissue was screened by gene chip technology,and the differentially expressed genes lnc RNA HOTTIP and RP1-261G23.7 were screened in order to explore their possible mechanisms in the pathogenesis of pterygium.Methods:Collected from June 2016 to May 2017 in the Yijishan Hospital of Wannan Medical College ophthalmology for surgical treatment of patients with pterygium(experimental group)and cataract extraction and intraocular lens implantation without pterygium patients(control group),20 patients(40 eyes in all)were enrolled in this study.The pterygium and normal nasal bulb conjunctiva tissues were collected during surgery.Twenty pterygium specimens(6 males,14 females,aged 47-84 years,mean age68.1 years old),normal nasal bulb conjunctiva tissue samples of 20 cases(9 males and11 females,aged 41-88 years,mean age 61.6 years old).Four samples of pterygium and four normal nasal bulb conjunctiva tissues were randomly selected from the tissue samples.The expression of lnc RNA in the experimental group and the control group was detected by high-throughput gene chip detection technique.The lnc RNA and their related target genes were significantly different;also randomly selected six tissue samples,the experimental group and the control group of three specimens by quantitative real-time polymerase chain reaction(q RT-PCR)verification Key lnc RNA HOTTIP,RP1-261G23.7 and corresponding target genes HOXA13,VEGFA,compared lnc RNA and target gene in the pterygium and normal nasal bulb conjunctival tissue whether there is a significant difference,and analysis of both target genes HOXA13,VEGFA Correlation of expression.Results:(1)The experimental group and the control group were selected by SPSS 17.0software for data processing.The gender and the age were analyzed by chi-square test.The statistical results were all> 0.05,with no statistically significant difference Interference with experimental factors;(2)Compared with normal nasal bulb conjunctiva tissues,8271 lnc RNAs were detected by high-throughput gene chip detection.Among them,lnc RNAs with P-value <0.05 and log fold change> 2 have 878 up-regulated genes and 1070 down-regulated genes.(3)From the lnc RNA microarray screening results,lnc RNAs with a high degree of variability were selected,and q RT-PCR method was used to verify the key lnc RNAs and target genes.The results showed that lnc RNAs and target genes were highly different between the experimental group and the control group(P<0.05,log fold change>2).(4)Two highly differentially expressed lnc RNAs and target genes,lnc RNA HOTTIP,RP1-261G23.7 and corresponding target genes HOXA13,VEGFA were verified by q RT-PCR with a high degree of consistency,significantly higher expression in normal nasal bulbous conjunctiva.Conclusion:(1)lncRNA is highly expressed in pterygium;Among them,lncRNA HOTTIP and RP1-261G23.7 showed the same result with the gene chip by q RT-PCR and had a differential expression in the pterygium;(2)HOXA13 and VEGFA target genes in the pterygiumare are higher than normal conjunctival tissues,both of which may play an important role in the pathogenesis and development of pterygium.