Preparation Of Residue Polysaccharide From Lentinus Edodes And Analysis Of Its Antioxidative, Anti-inflammatory And Pulmonary Protective Effects

Residues are the leftovers produced after the cultivation of edible fungi and contain bioactive substances such as polysaccharides.Recently,as the continuous expansion of the mushroom cultivation scale,more and more residues were discarded optionally,which not only caused a great waste of resources,but also led the environmental pollution.It was more and important to use residues rationally.In this study,Lentinula edodes residue polysaccharides(LRP),acidolysis Lentinula edodes residue polysaccharides(Ac-LRP),and enzymolysis Lentinula edodes residue polysaccharides(En-LRP)were used as the research object to analyze the antioxidative capacity in vitro,the contents of various inflammatory cytokines of bronchoalveolar lavage fluid(BALF),various indicators of serum,and antioxidant enzyme activity of lung tissue of acute lung injury(ALI)induced by lipopolysaccharide(LPS).Besides,the bond type,monosaccharide composition and purification of polysaccharides from Lentinus edodes also were researched.The results are as follows:(1)The three polysaccharides were separated and purified by DEAE-52 cellulose anion exchange column chromatography to obtain different polysaccharide components.The LRP had two components,Ac-LRP had one polysaccharide component and En-LRP had two components.The results of gas chromatography(GC)experiment showed that LRP contained rhamnose,arabinose,mannose,galactose and glucose in mass fraction of 12.96%,27.36%,3.64%,9.81%,and 46.23%,Ac-LRP contained rhamnose,arabinose,galactose,and glucose in mass fractions of 32.47%,22.53%,8.30%,and 36.70%,En-LRP contains rhamnose,arabinose,mannose,galactose,and glucose in mass fractions of 81.97%,4.63%,2.93%,4.75%,and 5.72%.Fourier transform infrared spectroscopy(FT-IR)analysis showed that the three polysaccharides all had the characteristic absorption peaks of polysaccharides.LRP was an α-glycosidically linked pyranose,and Ac-LRP and En-LRP were β-glycosidically linked pyranose.(2)In vitro antioxidant capacities of LRP,Ac-LRP and En-LRP were measured using reducing power,hydroxyl radical,superoxide anion radical,and DPPH radical as indicators.The results showed that as the concentration of bacillary dysentery increased,the antioxidant capacities gradually increased,showing a clear dose-dependent relationship.At the same concentration,En-LRP had the strongest reducing power and free radical scavenging ability.(3)The ALI mice model was established by intraperitoneal injection of LPS.The antioxidant,anti-inflammatory,and lung protective abilities of LRP,Ac-LRP and En-LRP were analyzed.The results showed that LRP,Ac-LRP and En-LRP could significantly reduce the wet/dry weight ratio,the myeloperoxidase(MPO)activity of lung tissue,the levels of three cytokines(TNF-α,IL-1β and IL-6)in BALF,the contents of complement 3(C3)and high-sensitivity C-reactive protein(hs-CRP)levels in mouse serum,and reduce the levels of malondialdehyde(MDA)and lipid peroxidation(LPO)in the lungs of ALI mice.At the same time,the activities of antioxidant enzymes(SOD,CAT,GSH-Px),and the totol antioxidant ability(T-AOC)of lungs increased significantly.In addition,morphological observations showed that the three residue polysaccharides,especially En-LRP,could significantly improve pathological changes in ALI mice.The results showed that LRP,Ac-LRP and En-LRP had anti-inflammatory and lung protective efficacy.The results indicated that LRP,Ac-LRP and En-LRP had certain anti-oxidative capacity in vitro and in vivo,and had a significant effect on the recovery of a series of lesions produced by LPS-induced lung tissue in ALI mice,opening up new research and development for new drugs.This research laid the foundation for the study of the structure-activity relationship of the pulmonary protective mechanism of LRP,Ac-LRP and En-LRP.