Study On The Function And Mechanism Of Long Non-Coding RNA Uc.38 In Breast Cancer

Breast cancer is the most common type of cancer in modern women.The incidence of breast cancer ranks first out of the various tumors in females worldwide,and the mortality rate currently ranks second.Although the mortality rate has declined with developments in medical science,the incidence and mortality of breast cancer are currently very high,particularly in many developed countries.Despite the huge threat to human heath,the molecular mechanism of breast cancer has not been clearly explained.Long non-coding RNAs are transcripts longer than 200 bp with no protein-coding capacity and they participate in human cancer development and occurrence.LncRNA can regulate transcriptional,epigenetical and post-transcriptional levels of gene expression.They have been proved to interact with nuclear acids and proteins to involve in a wide range of biological and pathological processes.Studying the molecular mechanisms of breast cancer occurrence and progression can helping establish effective prevention and treatment programs that can greatly help improve their live quality and make better long-term survival of patients.In our present study,the expression of uc.38 was down-regulated in both breast cancer tissues and breast cancer cell lines.Based on the results of in vitro and in vivo experiments,up-regulation of uc.38 expression inhibited breast cancer cell proliferation and induced cell apoptosis.And knockdown of uc.38 led to a significant increase in breast cancer cell proliferation.Thus,uc.38 suppressed breast cancer.Additional experiments revealed that uc.38 negatively regulated the expression of the pre-B-cell leukaemia homeobox 1(PBX1)protein and subsequently affected the expression of B-cell lymphoma-2(Bcl-2)family members,ultimately inducing breast cancer cell apoptosis.So uc.38 suppressed breast cancer cell growth by repressing PBX1 expression.Describing the uc.38/PBX1 axis has improved our understanding of the molecular mechanisms involved in breast cancer apoptosis and has suggested that this axis is a potential therapeutic target for breast cancer.