Study On Acute And Chronic Toxicity Of The Scolopendra Multilan's Finest Powder

Objective:Chinese medicine Scolopendra multilan is a traditional toxic animal medicine,and its most commonly used powder is the Scolopendra multilan's finest powder,but the toxic components in the Scolopendra multilan cause liver and kidney dysfunction,nervous system poisoning and anaphylaxis.The acute toxicity in mice and chronic toxicity test for 3 months in rats was used to explore the toxic dose,toxic reaction and possible toxic target organ of the Scolopendra multilan's finest powder,which aims to provide scientific experimental basis for clinical medication and determine the safe dosage.Methods:?1?Acute toxicity test in mice:the results of pre test found Dm?100%lethal dose?of the Scolopendra multilan's finest powder was 12.00g/kg,Dn?0%lethal dose?is 6.00g/kg,if the number of experimental groups is 6groups,calculation of measurement ratio r=1.148,thus the formal experimental measurement is determined as 6.00,6.89,7.91,9.08,10.43,12.00 g/kg.120Kunming mice were randomly into 6 groups,20 mice in each group,male and female,followed by the number of picric acid,with 0.30 g/ml,0.261 g/ml,0.227 g/ml,0.198 g/ml,0.172 g/ml,0.15 g/ml 6 concentrations of the Scolopendra multilan's finest powder in 0.4ml/10g intragastric administration in 24 hours.14 days was observed continuously from the drug administration and recorded:behavior,mental state,hair color,drinking water,and urine and stool in mice,there were abnormal exudates and death cases in the nose,eyes and mouth,the toxic reaction was recorded,including the symptoms,severity,onset and duration of poisoning,whether it was reversible or not.According to the death of mice,the median lethao dose(LD₅₀)was calculated.?2?Chronic toxicity in rats for 3 months:120 SD rats,male and female,7 days after feeding in the experimental environment,it was randomly divided into 4 groups:the high group,the middle dose group,the low dose group and the negative control group were 30 rats in each group.Continuous perfusion with different doses of the Scolopendra multilan's finest powder?0.5 g/kg,1.0 g/kg,2.0kg,10 times,20 times,40 times of the common clinical dose,respectively?and equal capacity of physiological saline.The medicine was given 1 times a day,5 days a week,and for 12 weeks,the hematology indexes?WBC,LYMPH,NEUT%,LYMPH%,MONO%,RBC,HGB,HCT,MCV,MCH,MCHC,RDW,PLT,PCT,MPV,PDW?,the blood biochemical criterion?TP,ALB,TBIL,AST,ALT,CK,TG,TCHO,BUN,CREA,Glu,K,Na,Cl?,the Viscera index?heart,liver,lung,kidney,thymus,brain,ovary,uterus,thyroid,adrenal,testis,epididymis?were measured at 4weeks,12 weeks of taking drugsand following observation for 2 weeks,in order to detect toxic target organs and organ histopathological examination.Result:?1?Acute toxicity test in mice:the symptoms of the mice in each group were similar,asexual difference of death in mice,Tetanic spasm,convulsion,incontinence,etc can also appear?especially in relatively high dose groups:9.08 g/kg,10.43 g/kg,12.00 g/kg?.Death of animals,liver,heart,spleen,lung,kidney and other maior organs,the naked eye has not been abnormal.Observation of 14 days after administration,the number and mortality of mice in each group were calculated by LD₅₀ data processing program,the Scolopendra multilan's finest powder is 8.8218g/kg of LD₅₀ in mice,the 95%confidence limit is 8.3800 g/kg?LD₅₀?9.2870 g/kg.?2?Chronic toxicity in rats for 3 months:the Scolopendra multilan's finest powder has a certain inhibitory effect on the weight growth of rats after sixth weeks of administration,and the recovery period is gradually restored;for 4 weeks,only RBC in 2.0g/kg group with the finest powder of centipede is lower than that of the negative control group,after 12 weeks of administration,the RBC,HGB decreased,PLT?PCT increased in the 0.5,1.0,2.0 g/kg groups of rats,but the recovery period could be restored to normal;for 4 weeks,the BUN and CREA in group 2.0 g/kg were higher than those in the negative control group,and there was no significant change in the other blood biochemical indexes,for 12weeks,the AST,ALT,CK,BUN,CREA in group 0.5,1.0,2.0 g/kg were higher than those in the negative control group,a decline in the recovery period,but the 2.0 g/kg group of CK,BUN,CREA still did not return to normal;the renal organ index of group 0.5 g/kg was lower than that of negative control group,and there was no dignificant change in the other organs;Histopathological examination,4 weeks of administration,and 1 case of mild renal fibrosis in group 2.0g/kg.12 weeks,?1?Renal disease:there was1 case of mild fibrosis in group 2.0g/kg,and 1 case of mild edema in group 0.5,1.0g/kg.?2?Liver disease:1 case of mild edema in group 1.0,2.0 g/kg.?3?Lung disease:1 case of mild inflammation in group 2.0 g/kg.?4?Testicular disease:1 case of coagulative necrosis of testis in group 2.0 g/kg.Convalescence,?1?Renal disease:there was1 case of mild fibrosis in group 1.0g/kg,1 case of mild inflammation in group2.0 g/kg and 1case of severe fibrosis.?2?Liver disease:1 case of mild edema in group 1.0 g/kg and group 2.0 g/kg.Conclusion:The damage of the Scolopendra multilan's finest powder to mice was mainly involved in the respiratory system,the nervous system,and LD₅₀ was 8.8218 g/kg,95%confidence limit is 8.3800 g/kg?LD₅₀?9.2870 g/kg,greater than 5000 mg/kg,belonging to the level of micro toxicity.The Scolopendra multilan's finest powder has a certain hemolytic toxicity,and toxic damage to the liver,kidney,lungs,heart and testicles in rats.