Analysis Of Pathogenesis Of Acute Myocardial Infarction Based On Correlation Of CircRNA-miRNA Network

BackgroundAcute myocardial infarction?AMI?is a type of severe cardiovascular disease with a high mortality and morbidity,sudden onset,and poses a great threat to human health.Early diagnosis and treatment of AMI can effectively reduce the mortality rate of AMI patients.How to improve the accuracy and timeliness of AMI diagnosis has become the focus of researchers.With the development of modern molecular science,the important role of circular RNA circRNA and micro RNA miRNA as non-coding RNA in the development of cardiovascular diseases has gradually attracted the attention of researchers;but circRNA and There is less research about whether miRNAs have mutual regulation in AMI.Therefore,the experimental design based on circRNA-miRNA network correlation to explore the pathogenesis of AMI.ObjectiveCirculating miRNA and miRNA gene expression profiles of AMI whole blood were analyzed using the Microchip? Capital Biotech Human CircRNA Array V2.0?4x180K?chip and the Agilent Human microRNA?Human miRNA Microarray Release 21.0?microarray to identify target genes that were significantly associated with AMI.,and coexpression analysis,and construction of circRNA-miRNA network regulation,to explore the relevance of circRNA-miRNA and its potential pathways to explore the pathogenesis of AMI;through FunDO?http://django.nubic.Northwestern.edu/fundo/?,NHGRI GWAS Catalog?http://www.genome.gov/gwastudies/?,KEGG pathway?http://www.genome.jp/kegg/pathway.html?,OMIM?http: / / ncbi.nlm.nih.gov/omim?and other databases perform bioinformatics analysis of the linear mRNA transcript corresponding to circRNA,confirming its exact relationship with AMI.MethodsAccording to relevant standards,10 cases of iliac artery blood samples were collected from AMI patients and control groups,and total RNA was extracted from them.Microarray microarray was used to analyze the gene expression profile of circRNA and microRNA,and the expression profile was analyzed by V1.0 gene expression microarray analysis system.Chip data processing and sorting,screening differentially expressed target genes;using miRanda software to predict miRNAs that may be bound by miRNA,constructing a circRNA-miRNA network map,analyzing and determining the circRNAmiRNA pathway to be verified next;the linearity corresponding to the differential circRNAs The mRNA transcripts were annotated with GO,disease,and pathway enrichment analysis to look for potential signaling pathways related to the pathogenesis of AMI.Results1.Compared with the control group,the circRNA expression profile in peripheral blood of AMI patients was significantly expressed,among which 1670 differentially expressed circRNAs,such as hsa-circ₁6316-10,hsa_circ₀140759,hsa_circ₀140760,hsa-circ₁6316-13,etc.?P< 0.05,fc?2?,13 differentially expressed mi RNAs,such as hsamiR-4299,hsa-miR-6832-5p,hsa-miR-1973,hsa-miR-4485-3p,etc.?P<0.05,fc ? 2?;2.circRNA-miRNA expression in whole blood of AMI group was network-related,in which hsa_circ₀099416,hsa_circ₀133145,hsa_circ₀139759,hsa_circ₀090876,hsa_circ₀023109 may regulate multiple signals through hsa-miR-6749-5p and hsa-miR-1202.Pathways are involved in the development of AMI;3.Databases of known metabolic and signal pathways and disease-related databases are used to annotate pathways,diseases,and functions of differentially expressed circRNAs.Disease enrichment analysis revealed five types of diseases closely related to the cardiovascular system.They were Hypertension,Hyperglycemia,Adiponectin levels,Stroke?Ischemic??Stroke?.?ischemic?)Coronary heart disease;no correlation pathway was found in the PANTHER database,P < 0.05;Insulin signaling pathway,Starch can be found in the KEGG PATHWAY database.Three related pathways were involved in the pathogenesis and disease progression of AMI,and sucrose metabolism?starch and sucrose metabolism?and Pentose phosphate pathway?P<0.05?.Metabolism of carbohydrates was found in the Reactome database.Multiple pathways such as metabolism,Glycogen storage diseases,and Signaling by VEGF?signal transduction?are involved in the development of AMI.4.GO analysis showed that its biological processes mainly involve cellular process,single-organism process,and metabolic process;cellular components mainly involve organelles and cells.Cell),membrane-enclosedlumen?membrane-enclosed lumen?,and cellpart?cell fraction?;molecular function mainly involves binding and catalytic activity.ConclusionCircRNA-miRNA is network-related and involved in the development of AMI.Among them,hsa_circ₀099416_CBC1,hsa_circ₀133145_CBC1,hsa_circ₀139759_CBC1,hsa_circ₀090876_CBC1,hsa_circ₀023109_CBC1 may regulate multiple signals through hsa-mi R-6749-5p and hsa-mi R-1202.Pathways participate in the process of AMI.