The Influence Of Astragalus Membranaceus On Calcium And Phosphorus Metabolism And FGF23-klotho Axis In Rats With Chronic Kidney Disease

Abnormal calcium and phosphorus metabolism is one of the most commonly complications in CKD patients.And with the development of chronic renal insufficiency,it is also evolved.The resulting complications including abnormal bone metabolism,calcification of blood vessel and soft tissue,pathological rarefaction of bone,and so on,which is serious harm to the quality of patients' lives and shortening their survival time.FGF23-Klotho axis regulate the metabolism of calcium and phosphorus in the body.Through adjusting FGF23-Klotho axis thus treating CKD-MBD is research hotspot in recent years.Traditional Chinese medicine(TCM)has its unique advantage in relieving chronic kidney diseases(CKD).In recently years,TCM gains more and more attention in terms of prevention and treatment of CKD and its complications.This paper is combined of contemporary literature research and the study of TCM in CKD,by means of animal experiment to explore the influence of astragalus membranaceus on calcium and phosphorus metabolism,FGF23-Klotho axis in CKD.Objective To observe the expression change of FGF23-Klotho axis on calcium and phosphorus metabolism in rats with chronic kidney disease,and the influence of Astragalus membranaceus on them.Methods 30 SD rats were randomly divided into three groups(control group,model group and AM group),after adaptive feeding for 1 week,250mg/kg adenine suspension by gastrogavage and 1.2%phosphorus content by dietary were executed to molding for 4 weeks.Then,adenine gavage was given once in 2d in the following 2 weeks;at the same time,the rats in AM group were given AM decoction 12.5g·kg-1I·d-1 gavage and control group&model group were given normal saline equivalent.Before dosing,selected 3 rats randomly in each group to detect the level of blood urea nitrogen(BUN),creatinine(Scr),calcium(Ca),phosphorus(P)via heart puncture blood.Meanwhile,the parathyroid hormone(PTH),FGF23,Klotho and 25(OH)D3 were detected by ELISA.After 6 weeks,the histological changes in renal were observed by HE staining,the level of BUN,Scr,Ca,P in serum were detected via abdominal aortic blood.Meanwhile,PTH,FGF23,Klotho and 25(OH)D3 were detected by ELISA.Results 1.1 Compared with the control group,the qualities of kidney and the BUN.Scr.Ca?P levels significantly increased in model group(,P<0.01,P<0.05),while the PTH level not markedly elevated.1.2 Compared with the model group,the qualities of kidney and the BUN?Scr?Ca?P levels were decreased in AM group,while the PTH level not markedly elevated,and the BUN?Ca?P levels were statistical difference(P<0.01,P<0.05).2.HE staining showed that the number of normal glomerulus improved in AM group compared with the model group,the expansion degree of renal tubular decreased,the number of inflammatory cells decreased,and the crystallization of adenine decreased.3.1 Compared with the control group,the FGF23 level increased in model group,while the Klotho and 25(OH)D3 decreased.3.2 Compared with the model group,the FGF23 level not markedly elevated in AM group(P<0.05),and the Klotho not markedly declined(P<0.05).Conclusion Astragalus membranaceus could alleviate the disorder of calcium and phosphorus metabolism in CKD rats,inhibit the high expression of FGF23,and up-regulate the expression of Klotho,thus improving histology of kidney,kidney function,and the balance metabolism of calcium and phosphorus in CKD rats.