| ObjectiveTo study the expression of HMGA2 and VEGF in colorectal cancer tissues and to analyze their correlation and clinical significance,and to explore its relationship with microsatellite instability(MSI).MethodsQualitative detection by immunohistochemistry:(1)The expression level of HMGA2 and VEGF in colorectal cancer tissues and paracancerous tissues;(2)The expression of MSI-related proteins MLH1,MSH2,MSH6 and PMS2 in colorectal cancer tissues.Semi-quantitative Western Blot detection of HMGA2 protein expression.Results1 、 The expression of HMGA2 and VEGF in colorectal cancer was significantly higher than that in paracancerous tissues(P <0.001);the expression level of HMGA2 was closely related to tumor differentiation and TNM stage(P <0.05)Diameter,depth of invasion,TNM stage and distant metastasis(P <0.05).2、There was a positive correlation between the expression of HMGA2 and VEGF in colorectal cancer(P <0.05).3、The incidence of MSI-H was correlated with the age,tumor location and differentiation of patients with colorectal cancer(P <0.05).The expression of HMGA2 was not related to microsatellite status(P> 0.05).The expression of VEGF in MSI-H group was lower than that in MSS / MSI-L group(P <0.05).Conclusion1 、 Colorectal cancer HMGA2 and VEGF were over-expressed,with clinicopathological features have a certain relationship,and the two are positively correlated,suggesting that both play an important role in colorectal cancer,synergistically promote the occurrence and development of colorectal cancer.2、HMGA2 expression has nothing to do with the status of microsatellites.The low expression of VEGF in MSI-H tissues may partly explain that MSI-H patients have a better prognosis than MSS/MSI-L patients. |
