| BackgroundAccording to the International Diabetes Federation(International Diabetes Federation,IDF)latest survey: 2017,about 425 million adults with Diabetes in the world,including China the number of people with Diabetes accounts for about a quarter in the number of Diabetes all over the world.China is a high incidence of diabetes,with a large base of diabetes.Statistics show that the risk of concurrent acute pancreatitis in diabetic patients is twice as high as that of normal people,and the risk of acute pancreatitis in young people with diabetes is greater.Diabetes is one of the most important disease of endocrine,immune system,and the intestinal tract is the body's largest emergency room,the number of intestinal flora,type is far more than is found,a growing number of studies have shown that intestinal flora and human obesity,cardiovascular disease and diabetes,especially type 2 diabetes and other metabolic diseases of the relationship between the development of the increasingly clear,the intestinal flora in play an important role in development and incidence of type 2 diabetes,in the intestinal flora of in-depth study of type 2 diabetes mellitus and acute pancreatitis is of great significance.Objective1.To clarify the changes of intestinal predominance microflora of type 2 diabetes mellitus with acute pancreatitis.2.Analysis of the relationship between intestinal microflora changes and intestinal pathological changes in rats with type 2 diabetes mellitus and acute pancreatitis.3.To explore the effect of intestinal flora changes on the prognosis of patients with type 2 diabetes mellitus with acute pancreatic injury.Methods1.The acute pancreatitis model of Wistar rats and the acute pancreatitis model of GK rats were constructed by using 3.5% sodium cholate solution retrograde bile duct injection.2.The modeling effect of random blood glucose and amylase activity was assessed by pathological section of the pancreatic tissue.3.The use of a variety of bacterial genome in tandem repeats ERIC(enterobacteria1 repetitive intergenic consen sussequences),ERIC-PCR fingerprint,compared with control group,the Wistar rats and GK rats acute pancreatitis 48 hours of intestinal bacteria community structure.4.Traditional bacterial culture method was used to observe the change of dominant microflora in rats with acute pancreatitis.5.The rat with successful model was made by staining the colonic pathological HE staining,and the pathological changes were observed.6.SPSS 22.0 software was used to carry out relevant statistical analysis of the data.Results1.AP group and DAP mortality rates were 20% and 40%,respectively,QB,DM group without death,pancreatic tissue pathology HE dyeing biopsy results show that the built module had obvious inflammation of rat pancreatic tissue infiltration phenomenon: in the pancreatic acini in inflammatory cells infiltration,acini have different degree of edema,hemorrhage and necrosis.2.24 h after measuring serum amylase: 1756.7±66.7AP group(P<0.05),1786.7±108.8 U/L DAP group(P<0.05),DAP,AP group have reached the pancreatic amylase average QB group of pancreatic amylase average more than 3 times,little difference comparing the two groups(P>0.05),no statistical significance;In DAP group,the random blood glucose level was 19.4±2.5mmol/L,and the AP group was 17.7± 2.0mmol /L,and the blood glucose of the DAP group was significantly higher than that in AP group,and the two groups were significantly different(P<0.05).3.ERIC-PCR fingerprint: compared with the DM group,the number of bands in DAP group decreased,the brightness of parts of 200bp-500 bp decreased,the brightness of 800-1100 bp band increased,and the diversity of intestinal flora was significantly different.The results showed that the QB group was significantly different from the other 3 groups,while the AP group and DAP group were close to each other,and there were some differences.4.The difference between the four groups was statistically significant.Further pairwise comparison showed that bifidobacterium and lactobacillus in DAP group were lower than QB group and AP group,and the difference was statistically significant(P<0.05).E.coli and denatured bacteria in DAP group were higher than those in QB group and AP group,and the difference was statistically significant(P<0.05).Conclusion1.The structure of intestinal flora after acute pancreatitis in rats with type 2 diabetes mellitus can change,which is shown as: the decrease of bacterial diversity;The decrease of probiotics(bifidobacterium and lactobacillus)and the increase of the opportunistic pathogens(e.coli and proteus);2.Compared with the pure acute pancreatitis group of type 2 diabetes mellitus and acute pancreatitis group in the rat intestinal flora diversity is lower and group four bacterial flora changes significant difference is compared between features are more obvious than simple acute pancreatitis group.3.Changes in intestinal flora may be involved in the progression of type 2 diabetes mellitus with acute pancreatitis by disrupting the intestinal microecological barrier and intestinal mucosal barrier. |
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