Modified Manufacture Of Rabbit VX2 Model Of Liver Cancer

Background:The VX2 liver cancer model is one of the most commonly used animal models for transcatheter arterial chemoembolization(TACE).There are several problems in making animal models:The single drug pentobarbital has poor anesthesia;Which of the open implants and open injections is better?Objective:In the preparation of the VX2 liver cancer model,The anesthetic effects of the two anesthetic methods were compared;and the pros and cons of two different intrahepatic tumor growth methods were compared.Methods:Sixty rabbits were randomly divided into four groups with 15 animals in each group.Group A was given a single anesthesia and an open injection method;Group B was given a single anesthesia and an open implantation method;Group C was given a compound anesthesia and open injection method;Group D was given a compound anesthesia and open implantation method.When using the same open injection method,the anesthesia induction time,operation time,resuscitation rate and anesthesia mortality rate of the single and combined anesthesia in group A and group C were compared;When the same kind of embedded planting method was used,the anesthesia induction time,operation time,resuscitation rate,and anesthesia mortality rate of single and combined anesthesia in groups B and group D were compared;When using the same type of single anesthesia,compare the operation time,incision length,operative mortality,intrahepatic tumor formation rate and extra hepatic ectopic implantation rate in group A and group B;When using the same compound anesthesia method,the operation time,incision length,operative mortality,intrahepatic tumor formation rate,and extrahepatic ectopic implantation rate in the group C and group D were compared.Results:1.Anesthesia induction time:The mean induction time of anesthesia was(19.4±0.9)minutes in the single group(group B),and(4.9±0.6)min in the composite group(group D).The average induction time of the anesthesia in the composite group(group D)was significantly lower than that of the single group(group B).(p<0.001).The difference was statistically significant.The mean anesthetic induction time was(18.2±1.5)minutes in the single group(group A),and(4.7±0.4)min in the composite group(group C).The average anesthetic induction time in the composite group(group C)was significantly lower than that in the single group(group A)(p<0.001).The difference was statistically significant.2.The average operation time:The average operation time was(35.0±5.7)minutes in the single group(group B),(20.6±1.0)min in the composite group(group D),and The average operation time was significantly lower in the composite group(group D)than in the single group(group B).(P<0.001).The difference was statistically significant.The average operation time of the single group(group A)was(33.1±6.7)minutes,the average operation time of the composite group(group C)was(18.9±1.1)minutes,and the average operation time of the composite group(group C)was significantly lower than that of the single group(group A).(p<0.001).The difference was statistically significant.3.Resuscitation rate:The resuscitation rate of single group(group B)was 40%,the resuscitation rate of composite group(group D)was 0,and the resuscitation rate of composite group(group D)was lower than that of single group(group B)(p<0.05);The difference was statistically significant.The resuscitation rate of single group(group A)was 40%,the resuscitation rate of composite group(group C)was 0,and the resuscitation rate of composite group(group C)was lower than that of single group(group A)(p<0.05).The difference was statistically significant.4.Anesthesia mortality:The anesthesia mortality rates in the single group(group A)and the composite group(group C)were 13.3%and 0%respectively,(p>0.05);in group A,only 2 cases died,and the difference was not statistically significant.There was no anesthetic death in the single group(group B)and the composite group(group D).5.Surgical mortality:The operative mortality rate was 7.7%in the injection group(group A)and 20%in the embedding group(group B),p>0.05.The difference was not statistically significantThe mortality rate was 13.3%in the injection group(group C)and 33.3%in the embedding group(group D),p>0.05.The difference was not statistically significant.6.The average operation time:The average operation time of the injection group(group A)was(33.1±6.7)min,the average operation time of the embedding group(group B)was(35.0±5.7)min,p>0.05.The difference was not statistically significant.The average operation time in the injection group(group C)was(18.9±1.1)min,the average operation time in the embedding group(group D)was(20.6±1.0)min,and the average operation time in the injection group(group C)was shorter than that of the embedding group(group D),p<0.001.The difference was statistically significant.7.Average incision length:Incision group(group A)average incision length was(2.5±0.2)cm,and embedding group(group B)average incision length was(3.5±0.1)cm.Injection group(group A)average incision length was shorter than the embedding group(group B),p<0.001;The difference was statistically significant.Incision group(group C)average incision length(2.5±0.1)cm,embedding group(group D)average incision length(3.5±0.1)cm.Injection group(group C)average incision length was shorter than the embedding group(group D),p<0.001.The difference was statistically significant.8.Liver tumor formation rate:Intrahepatic tumor formation rate was 100%in the injection group(group A);intrahepatic tumor formation rate in the embedding group(group B)was 91.7%,p>0.05;The difference was not statistically significant.The extrahepatic ectopic implantation rate in the injection group(group C)was 92.3%,and the extrahepatic ectopic implantation rate in the embedding group(group D)was 100%,p>0.05.The difference was not statistically significant.9.Extrahepatic ectopic implantation rate:The rate of extrahepatic ectopic implantation was 83.3%in the injection group(group A),16.7%in the embedding group(group B).and the extrahepatic ectopic implantation rate was higher in the injection group(group A)than in the embedding group(group B),p<0.05;The difference was statistically significant.In the injection group(group C),the extrahepatic ectopic implantation rate was 69.2%.In the injection group(group D),the extrahepatic ectopic implantation rate was 20%.The extrahepatic ectopic implantation rate of the injection group(group C)was higher in the embedding group(group D),p<0.05.The difference was statistically significant.Conclusion:1.In the preparation of rabbit VX2 liver cancer model,pentobarbital sodium combined with Simoimingxin II had better anesthesia effect than pentobarbital sodium single.2.The embedded planting method is superior to the injection planting method.