Intervention Study Of Grape Seed Proanthocyanidins Extact On Mild Cognitive Impairment

ObjectiveThe effect of Grape Seed Proanthocyanidin Extract(GSPE)on cognitive function and related serological markers in elderly patients with Mild Cognitive Impairment(MCI)was observed by the intervention study of GSPE on MCI.It provides scientific evidence for the application of GSPE and intervention strategies for the elderly with MCI.Method1.Volunteers were screened by Montreal Cognitive Assessment Scale(MoCA),in three communities in Huangshi City,Hubei Province,and determined for the intervention population according to the MCI diagnostic criteria,inclusion criteria and exclusion criteria.2.The study was conducted with a randomized,double-blind,controlled design experiment.Using the random number table method,the included cases were randomly divided into experimental group and control group.The experimental group received GSPE(320 mg/day).The control group received placebo with the same appearance and quantity,and the intervention period was 6 months.Cognitive function and serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL-C),low-density lipoprotein(LDL-C),glutathione reductase(GSH-PX),Interleukin-6(IL-6)and Human Silencing Protein 1(SIRT 1)levels were measured before and after intervention.3.All experimental data were established by Epidata 3.2 database,using SPSS16.0 for statistical analysis.Differences of observed indicators before and after intervention and differences between the two groups after intervention were analyzed.Result1.There are no significant difference in age,gender,BMI,marriage,hypertension,diabetes,stroke,history of traumatic brain injury,cultural level and MoCA score between the experimental group and the control group at baseline(p>0.05),which show that the two groups are comparable.2.Intra-group comparison before and after intervention,the MOCA scores of the subjects in the experimental group and the control group both increased significantly(p<0.05);after 6 months intervention,the MOCA scores of the intervention group increased(2.27 ± 3.26)higher than the control group(1.28 ± 2.81),but has no statistically significant(p>0.05).3.Intra-group comparison before and after intervention,serum TC and TG levels of the experimental group decreased,and the difference is statistically significant(p<0.05).The levels of HDL-C and LDL-C have no change(p>0.05).There are no significant difference in the levels of TC,TG,HDL-C,and LDL-C within the controlgroup(p>0.05).Comparison between groups,the level of TG has statistical significance after 6-month intervention(p<0.05),but there are no significant difference in the levels of TC,HDL-C,and LDL-C(p>0.05).4.After the intervention,GSH-PX,IL-6 and SIRT 1 have no difference in intra-group comparison and between-group comparison of experimental group and the control group(p>0.05).Conclusion1.GSPE has no improvement on the cognitive function of MCI patients during6-month period.It may be related to the smaller intervention dose and shorter intervention period.2.GSPE can regulate blood lipid levels in patients with MCI,especially with TG,which suggested a potential preventive effect on patients with vascular cognitive impairment.3.There is no observed effect of GSPE on antioxidant index(GSH-PX),inflammatory(IL-6)and related proteins(SIRT 1)in the serum of MCI patients,which are not inconsistent with the existing research results.Studies with larger doses and longer intervention periods are needed.