Study On The Obesity Model Of Zebrafish Induced By Environmental Endocrine Disruptors

Objective:Environmental endocrine disruptor(EEDs)was established to induce the animal model of zebrafish obesity.Methods:The acute toxicity of three EEDs to zebrafish was tested,the maximum exposure concentration was determined,and the homogeneity and stability of three EEDs in water concentration were tested.Through the induction of three substances,zebrafish larvae are obese.According to body mass index(BMI),triglycerides(TG),and other physiological and biochemical indexes,and connecting with the wood grain-eosin stain(HE)staining,oil red O staining two pathological index,comprehensive judgment EEDs induced obesity in zebrafish model was successful.Results:TBT exposed the LC50 of 96 h to 1.91 g/L,and the confidence interval of 95%was 1.37-2.65 g/L.DEHP's LC50 is>400 g/L;The LC50 of BPA is>100 g/L.In the course of the study,three kinds of substances were measured evenly and stable in water.The maximum induced concentration of the three substances was TBT 50ng/L,BPA 100 g/L,DEHP 200 g/L,and 3 doses of each substance were induced.In the study of induced obesity,there was a significant increase in BMI and TG in the three substances,the high dose group and the blank control group,and the HE staining showed that some animals in the high-dose group had hepatic steatosis.The results showed that the liver staining of the three substances in high dose group was significantly darker than that of the blank control group.Conclusion:TBT,BPA and DEHP were found in 25ng/L,50 g/L and 100 g/L concentrations,and continued exposure to 7-30dpf zebrafish larvae,which could cause abnormal lipid metabolism.The success of zebrafish obesity model was established by means of BMI,TG combined with HE staining and oil red O staining.