Preparation And Performance Study Of Silk Fibroin/Graphene Oxide Composite Hydrogel

Objective:In the present study,the hydrogel has become one of the most admirable material of tissue engineering scaffold,because of its polymeric networks strcture and hydrophilia which are beneficial to the matrix cell and tissue.Regretfully the problem of poor mechanical strength and short biodegradation time is generally found in medical hydrogel materials.Thus,a new model of porous scaffolds for tissue engineering was described.Graphene oxide(GO)was brought in to preparation a composite hydrogel based on silk fibroin(SF)by using the crosslinking:,1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDC)and N-Hydroxysuccinimide(NHS),with the purpose of modifying both of the mechanical property and degradability.Methods:We divide the hydrogels into few groups by means of the ratio change of GO/silk fibroin in which one is changed the concentration of GO and the others is silk fibroin.A series of the physical and chemical properties was investigated and the optimal ratio has been gated.Scanning electron microscope(SEM)was observed to find out the microstructure of the composite hydrogels;X-Ray Diffraction was tested to obtain the chemical bonding.Universal materials tester was used to measured the mechanical property.Besides,degradation testing was done according the protease XIV which was prepared for the mimetic body fluid and the losing weight was record respectively at 2?4?6?24?48?72?96?120hour.Results:Hence the composite hydrogel could meet the standards both in the strength of tissue regeneration and biological characterictics.The composite hydrogels prepared by EDC/NHS showed random coils according X-ray diffraction(XRD)and three-dimensional porous by scanning electron microscope(SEM)was successfully observed and the formation time could be shorten by adding the GO.Moreover,hydrogels exhibited both of favorable elasticity and compressive strength up to 130% When the concentration of SF and GO content were respectively 5wt% and 0.2wt%,the mechanical properties were the best.Otherwise,the addition of GO could significantly prolong the degradation time,and the loss rate of the quality can be reduced at least 10% in the same time.When the concentration of SF and GO content were respectively 5wt% and 0.2wt%,the degradation rate was the slowest,and the quality loss rate at 96 hours was only 11.74%.Hence the composite hydrogel will be considered valuable to future tissue engineering application.Conclusion:A newly SF/GO composited hydrogel,which was demonstrated to possess both favourable mechanical property and proper degradation was developed in this work,simply by the use of crosslinking.Hence it would be more suitable for proliferation and differentiation in cell culture and could be a potential material for the tissue engineering.