Study On The Preparation And Properties Of Graphene Oxide/Chitosan/Silk Fibroin Composite 3D Bone Tissue Engineering Scaffolds

Objective:To study the preparation and physicochemical properties of graphene oxide/chitosan/silk fibroin composite three-dimensional bone tissue engineering scaffolds,and hope to screen out bioscaffold materials with excellent performance for bone tissue engineering.Method:We prepared chitosan solution,silk fibroin solution and graphene oxide solution respectively,and we prepared mixed solution according to the mass ratio of chitosan solution and silk fibroin 1:1,the mass fractions of graphene oxide were at a ratio of 0%,0.5%,1%,1.5% and 2%.Named them 0% group(control group),0.5%group,1% group,1.5% group,and 2% group,respectively.By freeze-drying technology,three-dimensional composite porous scaffolds with stable physical and chemical properties were prepared.We observed the microscopic morphological characteristics of the five groups of stents using scanning electron microscope,measured their pore size and porosity,measured the stent porosity using the pycnometer method,used the PBS solution to determine the stent swelling rate and used the contact angle measuring instrument to measure the contact angle to determine the stent's Hydrophilic.Results:The surface and shallow structure of the scaffold materials with different concentrations of Go were observed by SEM.It was found that the interior of the stent was interpenetrating and different pores with different sizes and the pore diameter was between 50-250 nm.As the concentration of graphene oxide increased,the pore diameter Gradually decreased,the pore size of each experimental group was significantly smaller than that of the control group(P <0.05).The pycnometer method was used to measure the porosity of the scaffolds with different proportions of graphene oxide.The experiment found that with the increase of the concentration of graphene oxide,the porosity of the three-dimensional scaffold decreased.Among them,graphene oxide 0.5% group,1% group,1.5% Compared with the control group,the porosity of the scaffold in the group was not statistically different,only the porosity of the scaffold in the 2% group was significantly lower than that of the control group(P <0.05).The contact angle of the material was measured with a contact angle measuring instrument,and it was found that the water dropletscontacting the brackets of the 1% group,1.5% group,and 2% group were instantly absorbed.Through the measurement of the swelling rate of the graphene oxide scaffolds with different proportions,it was found that with the increase of the graphene oxide content,the swelling rate of the three-dimensional scaffold showed a decreasing trend,and the 1% group,1.5% group,and 2% group were significantly lower than the control group(P <0.05),there was no statistically significant difference between the0.5% group and the control group.Conclusion:The chitosan and silk fibroin solution after adding graphene oxide can obtain a scaffold with a porous structure and communicating with each other between pores with stable physical and chemical properties by freeze-drying method.The scaffolds containing 1% and 1.5% graphene oxide perform well in terms of pore size,porosity,hydrophilicity,and stability,and can be used as a bone tissue engineering scaffold material for further experimental research.