Effects Of Heavy Ion Beam And X-irradiation On The Proliferation,Migration,Invasion And Related Mechanisms Of Human Tongue Squamous Cell Carcinoma CAL27 Cells

Objective:To study the biological effects and its molecular mechanism of human tongue squamous cell carcinoma CAL27 cells after heavy ion(¹²C⁶⁺)beam and X-irradiation.Method:The MTT?3-?4,5-dimethylthiazol-2?-2?colorimetric method was used to detect the cells after irradiation with different doses of heavy ion beams(¹²C⁶⁺)and X-rays on human tongue squamous cell carcinoma CAL27 cells.Proliferation ability under different irradiation conditions and doses;Cell migration ability under different irradiation conditions and doses was detected by Transwell migration and scratch test;Cellular sensitivity to radiation under different irradiation conditions and doses was tested by cloning experiments.Transwell chamber invasion assay was used to study the invasive ability of CAL27 cells under different irradiation conditions and doses;Western Blot was used to observe the expression of NF-?B-P65 protein in CAL27 cells with different doses of heavy ion beam(¹²C⁶⁺)and X-rays.Impact.Result:1.The inhibitory effect of heavy ion(¹²C⁶⁺)and X-irradiation on the proliferation of CAL27cells was positively correlated with dose-time.2.after 24 h irradiation of CAL27 cells with 0 Gy,1 Gy,2 Gy,4 Gy of different doses of heavy ions(¹²C⁶⁺)and X-rays,the migration inhibitory rates of the carbon ion irradiation group were:0%,34.10%,45.46%and 53.995%?0Gy of unirradiated cells served as control group,P<0.05?;the scratch mobility of scratched test cells was 50.12%,47.17%,35.03%,and 21.99,respectively,in the carbon ion irradiation group 36 h after irradiation.%?0Gy of non-irradiated cells as a control group,P<0.05?;and the cell migration inhibition rates of the X-ray irradiated group were:0%,-8.41%,-30.03%,and-44.77%?0Gy of non-irradiated cells.As a control group,P<0.05?.The scratch cell migration rate of the scratch test cells were:-0.62%,-7.16%,-10.23%,and-17.84%?0Gy of unirradiated cells was used as a control group,P<0.05?.3.After 14 days irradiation of CAL27 cells with 0 Gy,1 Gy,2 Gy,4 Gy different doses of heavy ions(¹²C⁶⁺)and X-rays,the survival fraction of clones in the carbon ion irradiation group was:100%,19.33%,respectively.4.38%and 1.03%?non-irradiated cells 0Gy served as control group,P<0.05?;while the X-ray irradiation group clone survival fractions were:100%,40.58%,19.51%and 4.93%?unirradiated cells 0Gy As a control group,P<0.05?.4.After 24 h irradiation of CAL27 cells with 0 Gy,1 Gy,2 Gy,and 4 Gy different doses of heavy ions(¹²C⁶⁺)and X-rays,the inhibition rates of carbon ion irradiation group were:0%,34.10%,45.46%and 53.995%?0Gy of non-irradiated cells served as a control group,P<0.05?;while the X-ray irradiation group had an invasion inhibition rate of 0%,-8.41%,-30.03%,and-44.77%?unirradiated?.Cells 0Gy served as a control group,P<0.05).5.Compared with the blank control group?0Gy cells not irradiated?,24h after irradiation,different doses of X-ray had no significant effect on the expression of NF-?B-P65 in CAL27 cells?P>0.05?;After exposure to heavy ion beam(¹²C⁶⁺)at time and dose,the expression of NF-?B-P65 in CAL27 cells was significantly decreased with the increase of irradiation dose?P<0.05?.Conclusion:Compared with conventional X-rays,heavy ion(¹²C⁶⁺)irradiation significantly inhibited the proliferation and migration of CAL27 cells with dose and time effects and had a higher biological effect.It provides a theoretical basis for the treatment of tongue cancer by heavy ion beam.