Fabrication And Characterization Of Fibrous Barrier Mat Of PLCL/SF Loaded With PTH-Fc

Objective: The aim of the present study was to fabricate fibrous barrier mat of poly(L-lactide-co-caprolactone)/silk fibroin loaded with PTH-Fc via electrospinning technique,and to study its hydrophily,biocompatibility,biodegradability,mechanical strength,and ability to regulate appropriate cellular activities.Methods: electrospun fibrous Poly(L-lactide-co-caprolactone)/silk fibroin loaded with PTH-Fc(PTH-Fc/PLCL/SF)at different parameter were prepared and compared with each other.Apparent morphology before and after degradation were observed by scanning electron microscope(SEM).Tensile property was tested by a custom mechanical testing device.Contact angle shows the hydrophilicity of membranes.Then we chosen the best parameter,and in vitro biodegradation and drug release behaviors were tested.The biocompatibility of PLCL/SF/PTH-Fc were evaluated by MTT and the cell adhesion assay.The osteogenic potential of the cell cultured with PLCL/SF/PTH-Fc was investigated by Alizarin red stain.Results: Our results suggests the nanofiber of PLCL/SF/PTH-FC were uniform and smooth.The average fiber diameter of most uniform group is 556.45 ± 78.9 nm.The diameters were increasing by increased voltage and decreased received distance.XRD shows electrospinning process,crosslinking agent and the addition of PTH-Fc have no effect on blended PLCL/SF.tensile strength and stiffness of electrospun fiber is increasing as diameter reducing.in this study,all our groups are hydrophobic.PTH-Fc/PLCL/SF mats can degrade and promote cell proliferation.In our study,drug burst release at the early time: about 60% of PTH-Fc was released from the nanoscaffold during the five days.And there was no statistically significant difference between experimental group and control group(P>0.05).But it can induce the cell differentiation.Conclusion: electrospun fibrous PLCL/SF/PTH-Fc has good mechanical property and biodegradability,although it is hydrophobic.It can promote cell proliferation and cell differentiation.