The Inhibitory Effect And Its Mechanism Of RGD Peptide On The Cancer Stem Cells Of Larynx

Objective: RGD peptide has the activity of inhibiting the proliferation of laryngeal cancer stem cells,but it is not clear that the inhibition of human laryngeal cancer stem cell and its potential molecular mechanism.This project aims to detect the expression of laryngeal cancer stem cells in alpha v beta 3,combined with RGD peptides,observe the inhibitory effect of laryngeal cancer stem cells,and participates in the tumor angiogenesis related molecular detection,explore its molecular mechanism,to explore the diagnosis and treatment of laryngeal cancer of new ideas and new methods.Methods: This study intends to select and cultivate human laryngeal cancer stem cells.To detect the expression of integrin in human laryngeal cancer stem cells;RGD peptide was cultured with laryngeal cancer stem cells to observe the effect of RGD peptide on proliferation and activity of laryngeal cancer stem cells.To determine the expression level of VEGF/ vegfr-2 /STAT3/ hif-1 in human laryngeal cancer stem cells,and to explore the mechanism of RGD peptide to inhibit laryngeal cancer stem cells.Results: 1.Cytotoxicity-proliferation experiments showed that the proliferation ability of laryngeal cancer stem cells after RGD peptide was decreased and the activity decreased.2.RT-qPCR and Western Blotting showed that in vitro,RGD peptide can inhibit angiogenesis signaling pathway related proteins at mRNA level and protein level.And the dose-dependent relationship is presented.3.The Nude mice tumor-bearing test showed that in vivo,RGD peptide can inhibit tumor growth by inhibiting angiogenesis.Conclusion: RGD peptide has an inhibitory effect on the stem cell of larynx cancer.Its mechanism is that the activation level of angiopoietin is decreased and the angiogenesis of tumor is reduced.