| Purpose To explore the impact of peritoneal metastasis of human gastriccancer cells by dextran sulfate (DS),investigate the influence of dextran sulfate on theexpression of integrin beta1and vascular endothelial growth factor（VEGF）,toinvestigate DS on the prevention and mechanism of implanting metastasis of gastriccancer cells in the abdominal cavity.Methods The nude mice108were randomly divided into experimental group(60nude mice) and control group (48nude mice), the control group and theexperimental group were randomly divided into five groups.All nude mice wereinjected of tumor cells by the way of0.2ml/only with the number of1x107cells inabdominal cavity in the same time, and the experimental group were injected of0.3%DS by the way of1ml/only, the control group given0.9%physiological saline by theway of1ml/only as a negative control. The nude mice were killed on the1st day afterinjecting, the2nd day, the3rd day, the7th day, the14th day respectively, and naturaldeath after. Observe the number of peritoneal cancer nodules by caesarean section andconfirmd by HE staining. Record the number of tumor nodule in abdominal cavity.Using the method of immunohistochemical、immunofluorescence and RT-PCR todetect the expression of integrin beta1and VEGF.Results With the extension of the implanting time of gastric cancer cells, incontrol group and experimental group, the number of mean tumor nodules gradually increased, and the expression of Integrin beta l and VEGF both gradually increased.The number of mean intraperitoneal metastatic nodules of the experimental group wassignificantly less than the control group, both P<0.001. Immunohistochemical resultsshow that the experimental group of integrin beta1and VEGF protein expressionsignificantly lower than the control group, both P <0.001.By the analysis of RT-PCR,the integrin beta l mRNA and VEGF mRNA of the experimental group weresignificantly less than those of the control group,both P<0.001.They are both positivecorrelation between integrin beta l and VEGF expression in gastric cancer tissuein thecontrol group and the experimental group.The survival time of the experimental groupsignificantly longer than control group in nude mice survival time. By the analysis ofRT-PCR, the integrin beta l mRNA and VEGF mRNA of the experimental group weresignificantly less than those of the control group in omentum majus, both P<0.001inthe control group and the experimental group.they are both positive correlationbetween integrin beta l and VEGF expression in gastric cancer tissuein the controlgroup and the experimental group.Survival time of experimental group nude micesignificantly longer than control group in nude mice survival time.Conclusion Firstly, DS can inhibit the planting metastasis of gastric cancercells in the greater omentum, can cut the expression of integrin beta1and VEGF atthe same time. Secondly, there is a positive correlation between the expression ofintegrin beta l and VEGF expression in gastric cancer tissue of omentum. Thirdly, DSmay inhibit the celiac planting metastasis of gastric cancer by cutting the expressionof integrin beta1and VEGF. fourthly, DS has the prevention function on the celiacplanting transfer of human gastric cancer cells... |
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