| At present, the sensors constructed by chemically modified electrodes are mainly divided into enzyme and non-enzyme type. Although the performance of enzyme sensor is good, it depends on enzyme too much; when the environment is not conducive to the existence of enzyme, the performance of the sensor is weakened. However, the type of non-enzyme sensor eliminates the limitation of the enzyme, which indicates it is not influenced by the external environment. Thus, the type of non-enzyme glucose sensor attracts more and more attention. In the meanwhile, with the application of nanomaterials, the performance of non-enzyme sensor gets greater improvement. In this thesis, the glassy carbon electrode will be modified by nano transition metallic oxide and its composites, aiming to obtain nano material modified electrode. Besides, non-enzyme glucose sensor will be constructed on the basis of the electrode, which will successfully detects glucose concentration. And the procedures are as follows:Firstly, this thesis synthesizes three kinds of Cu O nanomaterials with different structurs, Graphite oxide(GO) and CuO/RGO respectively through the hydrothermal and hummers method, and analyzes the material's phase composition and morphology by XRD and SEM; Secondly, namometer CuO with different structure will be modified in the surface of glassy carbon electrode; In alkaline conditions, this thesis makes the contrastive study of the performance of glucose detection of CuO with modified different structures of electrodes. Accordingly, the experiment discovers that electrodes of the spherical nano CuO with muti-level structure(CuO-NSs/Nafion/GCE) have superior performance compared with lamellate(CuO-NLs/Nafion/GCE) and granular nano CuO electrodes(CuO-NGs/Nafion/GCE); Finally, CuO/RGO will be modified in the surface of GCE, aiming to achieve CuO/RGO/Nafion/GCE. By electrochemical experiment, it is found that the performance of glucose detection of CuO/RGO/Nafion/GCE is better than CuO/Nafion/GCE. |
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