| The non-enzymatic glucose sensors can overcome the problem that the enzymaticglucose sensors lack of stability, the glucose oxidase （GOD） can easily be affected bytemperature, pH value, and toxic chemicals. The non-enzymatic glucose sensors haveattracted much research interest, such as electrochemistry and electro-analyticalchemistry. To improve the stability and analytical performance and reduce theinterference of the sensors, convenient methods were investigated in the preparation ofnon-enzymatic glucose sensors. Six kinds of nanoparticles including Cu（OH）2/PPyox,Co（OH）2/PPyox, Ni（OH）2/PPyox, Ni（OH）2/MWNT, Cu-Ni（OH）2/PPyox, Pt-Ni（OH）2/PPyox were modified on carbon ceramic composite electrodes （CCE） by usingelectrochemical methods. The electrochemistry activity of the glucose was investigatedon the above sensors and established the electrochemical detection methods. Theresearches broaden the analytical chemistry of non-enzymatic glucose sensors. Theresearches possessed of potential application values in improving the stability andbroaden the electroanalytical chemistry of non-enzymatic glucose sensors. This thesismainly studied the preparation and electrochemical activity of the followingnon-enzymatic glucose sensors. The main contributios of this dissertation weresummarized as follows:1, The non-enzymatic glucose sensors of Cu（OH）2/PPyox/CCE, Co（OH）2/PPyox/CCE, Ni（OH）2/PPyox/CCE were fabricated by using cyclic voltammetry. First, theOverxidized polypyrrole electrode was fabricated by using the single potential step orcyclic voltammetry. Second, the MHCF/PPyox/CCE was fabricated from the solutioncontaining Mn+, EDTA and KHCF by using cyclic voltammetry. Then the metalhydroxide film on the surface of the sensor was obtained by electrochemical conversionfrom metal hexacyanoferrate in0.3mol·L-1NaOH solution. The electrocatalytic activity of the glucose were investigated on the non-enzymatic glucose sensors ofCu（OH）2/PPyox/CCE, Co（OH）2/PPyox/CCE, Ni（OH）2/PPyox/CCE. The experimentalresults showed that the non-enzymatic glucose sensors exhibited stongly electrocatalyticactivity towards the oxidation of glucose. Under the optimal conditions, the oxidationpeak current showed the linear relationship with the concentration of Glucose on theCu（OH）2/PPyox/CCE, Co（OH）2/PPyox/CCE, Ni（OH）2/PPyox/CCE were obtained theseparate ranges of2.010-75.610-4mol·L-1(s=2500.0μA·mM-1·cm-2) and5.610-41.210-3mol·L-1(s=1250.0μA·mM-1·cm-2),5.010-73.610-4mol·L-1(s=2774.9μA·mM-1·cm-2) and3.610-42.210-3mol·L-1(s=1661.5μA·mM-1·cm-2),2.0×10-71.7×10-4mol.L-1(s=3047μA.mM-1.cm-2) and1.7×10-42.7×10-3mol.L-1(s=1380μA.mM-1.cm-2), with the detection limit of （3sb）1.010-7mol·L-1and1.010-7mol·L-1and5.0×10-8mol.L-1. The proposed methods were applied to detect Glucose inthe serum.2, The nano-NiHCF/MWNT was fabricated in the solution containing theK3Fe（CN）6, Ni（NO3）2and MWNT by using layer-by-layer. Then droplet spreads on thecomposite ceramic carbon electrode （CCE） surface, preparation the non-enzymaticglucose sensor of the NiHCF/MWNT/CCE. The NiHCF/MWNT/CCE was placed in theNaOH solution, using cyclic voltammetry scan activation, the nickel hexacyanoferratesderived from nickel hydroxide, obtained the non-enzymatic glucose sensor ofNi（OH）2/MWNT/CCE. The electrochemical behavior of glucose was investigated on theNi（OH）2/MWNT/CCE. The result showed that: the non-enzymatic glucose sensorsexhibited strongly electrocatalytic activity towards the oxidation of glucose. Thecalibration curve was over the range of2.0×10-75.7×10-4mol·L-1(s=2786.5μA·mM-1·cm-2) and5.7×10-42.7×10-3mol·L-1(s=2005.2μA·mM-1·cm-2) with adetection limit of8.0×10-8mol·L-1, the proposed method was applied to detect Glucosein the serum.3, Copper and nickel oxide composite films modified sensor was electrochemicaldeposited on PPyox/CCE using cyclic voltammetry in the solution containing Cu2+and Ni2+. Then the Cu-Ni（OH）2/PPyox/CCE was fabricated by electrochemical conversion.The electrochemical behavior of glucose was investigated on the Cu-Ni（OH）2/PPyox/CCE. The result showed that: the non-enzymatic glucose sensors exhibitedstrongly electrocatalytic activity towards the oxidation of glucose. The calibration curvewas over the range of5.0×10-71.1×10-3mol·L-1(s=1069.3μA·mM-1·cm-2) and1.1×10-3mol·L-15.8×10-3mol·L-1(s=505.7μA·mM-1·cm-2) with a detection limit of2.0×10-7mol·L-1（3sb）, The proposed method was applied to detect Glucose in the serum.4, A nano-Pt-Ni（OH）2/PPyox/CCE was fabricated by using cyclic voltammetry. Theelectrochemical behavior of glucose was investigated on the Pt-Ni（OH）2/PPyox/CCE.The result showed that: the non-enzymatic glucose sensors exhibited stronglyelectrocatalytic activity towards the oxidation of glucose. The calibration curve was overthe range of2.0×10-75.7×10-4mol·L-1(s=4125μA·mM-1·cm-2) and5.7×10-42.7×10-3mol·L-1(s=2923μA·mM-1·cm-2) with a detection limit of8.0×10-8mol·L-1（3sb）,the proposed method was applied to detect Glucose in the serum. |
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