Study On The Extraction Process Of Astragaloside From Astragalus Membranaceus And The Preparation Of Astragalus Membranaceus

Astragalus armour glycosides can regulate blood viscosity reduce thrombosis,so as to achieve lower blood pressure and protect the heart,the purpose of a back injury of free radicals,cancer prevention,anti-aging,improve hypoxia,enhance immunity function.In yulin guangxi province were investigated based on the root of remembranous milk vetch yinpian?producing area,gansu province,and bao tong Chinese medicine yinpian co,LTD,production?as raw material,with astragalus glycosides content for clues,supercritical CO₂ extraction,accelerated solvent extraction method and reduced pressure within the boiling method,the optimum extraction process conditions,three methods compared with type extraction respectively,to determine the best extraction method.Then by using the method of optimizing process to extract effective components of radix astragali,USES the wet legal system grain of astragalus particles prepared by and evaluate its quality.?1?Soxhlet extraction method from astragalus extract astragalus glycosides,respectively by HPLC ELSD and HPLC-CAD method determination of the content of astragalus armour glycosides in the same sample,the results showed that the HPLC ELSD and HPLC-CAD can accurately and reliably to content determination of astragalus armour glycosides,HPLC-CAD has higher sensitivity and reproducibility,so using electrospray test analysis of astragalus membranaceus methyl glucoside content in radix astragali.?2?Astragalus extraction by supercritical CO₂ extraction of astragalus glycosides.With different concentrations of ethanol?50%,60%,70%,80%?as an entrainer,in the case of other conditions unchanged,to extract content determination,the results with concentration of 70%ethanol as entrainer extract astragalus armour glycosides have the highest rate.With concentration of 70%ethanol as an entrainer,the main factors affecting the extraction pressure,extraction temperature,extraction time and velocity of four factors in the orthogonal experiment,the results showed that the supercritical CO₂ extract astragalus astragalus armour glycosides in yinpian optimization conditions as follows:70%ethanol as an entrainer,40 MPa extraction 50 ? temperature,extraction pressure,extraction time for 1 h,extraction velocity of 25?1.h-1?.In the accelerated solvent extraction method to extract astragalus astragalus glycosides.With water saturation n-butyl alcohol and methanol as extraction agent,in the case of other conditions unchanged,to extract content determination,the results of water saturation n-butanol extraction yield higher than that of methanol.With water saturation n-butyl alcohol as extracting agent,choose the main factors affecting the extraction temperature,time and the number of cycle 3 factors such as orthogonal test,the results showed that astragalus armour glycosides in ASE extraction of astragalus membranaceus yinpian optimization condition for:water saturated n-butyl alcohol as static extraction solvents under 100 ? for 10 min,cycle number 3 times.Decompression internal boiling method of extracting astragalus astragalus glycosides.With methanol and ethanol as resolution agent,in the case of other conditions unchanged,to content determination of extract,the extracted by methanol as resolution agent astragalus armour glycosides high yield.With methanol as the desorption agent,select the main factors affecting the extraction temperature,concentration of solvent and extraction time on 3 factors in the orthogonal experiment,the results show that the reduced pressure within the boiling method of extracting astragalus astragalus armour glycosides in yinpian optimization condition for:with methanol as the desorption agent,concentration of 70%,extraction under 70 ? for 5 min.Three methods were used respectively to the optimization of conditions in the extraction of astragalus astragalus glycosides,and compared with type extraction.Results astragalus extraction by supercritical C02 extraction slices of astragalus glycosides average of 0.121%,ASE in the method of extracting astragalus membranaceus yinpian astragalus glycosides average of 0.127%,reduced pressure within the boiling method of extracting astragalus slices of astragalus glycosides average of 0.089%,soxhlet extraction method to extract astragalus slices of astragalus glycosides average of 0.090%.According to yield effective components,extraction time and solvent consumption factors such as comprehensive analysis,that accelerated solvent extraction method to extract astragalus astragalus glycosides plant a is much better than other methods of slices,so make sure the method of extracting astragalus armour glycosides by accelerated solvent extraction method.?3?Using accelerated solvent extraction method the active components of extraction of radix astragali,then astragalus extract made from thick cream,sugar powder and dextrin as excipient,ethanol as the wetting agent,with granules solubility,formability,hygroscopicity,viscosity as a preparation for molding process evaluation index,select the best raw material proportioning extract:sugar powder,dextrin as 1:3:1.According to the granule preparation technology preparation of astragalus particles.In astragalus particles quality inspection process,with the variation in content of astragalus armour glycosides as the clue,using high performance liquid chromatography-electrospray assay?HPLC-CAD?to determine its content,in order to investigate the stability of astragalus particles and some influence factors of the influence of the contents of astragalus armour.Results show that each index of astragalus particles all conform to the requirement of pharmacopoeia,the stability of heat and light is good,the environment where the temperature 60 ?,humidity 80%place after one month,determination of its content to check compliance,showed that astragalus particles in well stability within a month.