The Preliminary Studies About The Extraction Conditions And The Metabolism In Vitro Of Saponin That In Astragalus Membranaceus (Fish.) Bge. Var. Mongholicus (Bge.) Hsiao

Astrgalus membranaceus(Fisch.)Bge. var. mongholicus(Bge.)Hsiao is famous-region drug in shanxi province. It can benefit vital energy in our body. Modern pharmacology studies confirmed that, Astrgalus membranaceus(Fisch.)Bge. var. mongholicus(Bge.)Hsiao has the following effect including:anti-virus infection, protecting the heart and kidney, Bi-directional regulation of blood sugar, Anti-hypoxia, anti-free radical oxidation, anti-aging, anti-tumor, enhance immunity, bi-directional regulatory role of the central nervous system, diuretic, hypertension, etc. Astragalus contains mainly astragalinⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴand many other saponins, calycosin, quercetin, isoflavans, formononetin and other flavonoids astragalus polysaccharideⅠand other polysaccharide components, and variours amino acids and trace elements. High-performance liquid chromatography has been widely used in quality control of traditional medicine due to its high sensitivity, UV detector has the characteristic of the high sensitivity and stability, and widely used in high performance liquid chromatography. But it can apply only to ultraviolet and visible light absorption substances. Because Many of substances, do not contain UV-absorbing group, they have no absorption of ultraviolet or visible light, or UV-visible light weak absorption, such as saponins, sugars, terpenoids, steroid, amino acids and fatty acids and other compounds, they are difficult to apply the HPLC-UV detector for detection. Evaporative light scattering detector's advanced characeristics bring high-performance liquid chromatography detection techniques energy, the main characteristics of this detector are that: the matter without UV-visible light absorption can be measured, application in the gradient elution, can work in not harsh temperature, and higher Sensitivity than a differential refractive index detector. ELSD do not rely on the optical properties of the sample and the ELSD detector is more suitable for such analysis and quality control of these traditional Chinese medicines.By way of HPLC-ELSD, This paper preliminary studies the extraction technology and the metabolism in vitro of saponins active ingredient in Astragalus membranaceus (Fisch.)Bge. var. mongholicus (Bge.) Hsiao.1. Study on analytical method for determination of the content of astragaloside IV in Astragalus membranaceus (Fisch.)Bge. var. mongholicus (Bge.) Hsiao.To establish analytical method that determines the content of astragaloside IV in Astragalus membranaceus(Fisch.)Bge. var. mongholicus(Bge.) Hsiao by used of HPLC-ELSD. The Diamonsil C18 column was used; and the mobile phase was a mixture of acetonitrile—water; temperature of column was 25℃; injection volume:20μL; ELSD detector drift tube temperature:60℃, gas flow:2.0L/min, gain:8. In the system Applicability Test, The column number of theoretical plates in accordance with terms astragaloside IV 351,083, separating degree was 4.23, tailing factor was 1.02, indicating a good symmetry, retention time of 35.58 min. By the study on linear, precision, stability, recovery, etc, the study results show that Linear range of astragaloside IV concentration was 7.5～750μg/mL R2= 0.9972, showing a good linear relationship, the RSD of precision was favorable, the mean recovery was between 98.0～102.0%, good stability and the HPLC-ELSD method established in this paper was simple for determination of astragoloside IV in Astragalus membranaceus (Fisch.)Bge.var.mongholicus (Bge.)Hsiao.2. Study on optimization of extraction process or Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)HsiaoTo optimize the extraction technology for Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)Hsiao dissolved in methanol-ammonia water. The process was studied by orthogonal design L9 (34) with the extraction rate of astragoloside IV as criteria. The optimum extracting condition was:adding amount of solvent (methanol:ammonia water is 9:1) ultrasound extraction for 40 min(totally extracting for 3 time).3. Study on metabolism in vitro in rat liver microsomes for Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)HsiaoIn this study, a preliminary study on metabolism of the extract of Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)Hsiao in rat liver microsomes by HPLC-ELSD, it has certain role in study on Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)Hsiao, Scientific understanding of the safety of Chinese medicine, Clinical medication, and safety evaluation etc.4. Study on the atlas of extract in Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)Hsiao and Radix Astragali InjectionIn this experiment, to study the atlas of extract in Astragalus membranaceus(Fish.) Bge.var.mongholicus (Bge.)Hsiao and Commercially available injection made of Astragalus using gradient elution by HPLC-ELSD method. The atlas show that, there are corresponding main component peaks, but the number of the peak and peak area ratio between them were not the same. Illustrated that the quality, stability, controllability of the traditional chinese medicine injection can be assured only on condition that a fixed place of origin of Raw medicinal herbs and extraction process control, provide certain scientific basis and reference for the studies of Astragalus herbs and injection products containing the main ingredient Astragalus.