Preparation Of Fluorescent Nanoparticles And Its Application In The Analysis Of Mitoxantrone Drugs And Heavy Metals

Fluorescent materials is a key factor in the fluorescence analysis method.Two aspects of the work were launched with fluorescent nanomaterials as the object of study in this paper.First,a mitoxantrone imprinted polymer ratiometric fluorescent probe was prepared,and the method was established for the determination of mitoxantrone in biological fluid.Second,an organic fluorescent nanomaterial was prepared and the determination method of Hg²⁺ in environmental water samples was established using the organic fluorescent nanomaterial as fluorescent probe.This thesis mainly includes the following four parts:?1?Introduction:A brief introduction including the classification of the fluorescent materials,synthetic methods,direction of their application fields are given.?2?Preparation and application of mitoxantrone imprinted polymer ratiometric fluorescent probe:The mitoxantrone imprinted polymer ratiometric fluorescent probe was prepared by sol-gel method,the method was established for the determination of mitoxantrone in biological fluid.The results of fluorescence experiments and transmission electron microscopy characterization showed that MIP@rQDs@SiO2 ratiometric fluorescent probe was successfully synthesized.The as-prepared MIP@rQDs@SiO2 ratiometric fluorescent probe could selectively recognize template MIT.The maximum adsorption capacity of the probe toward MIT reached 6.81 mg·g^-1,which was 5.4 times that of the non-imprinted probe.Methodological study results showed that a good linear relationship between analytical signal?I52a/I62a?0/?I524/I62a?and CMIT when MIT concentration at the range of 0.2-2.0?mol·L^-1,with the linear equation being y=0.9581+0.5499x and correlation coefficient R2=0.9923.The detection limit of MIT was 0.067 ?mol·L^-1,the imprinting factor was 30.02.The sensitivity was higher than that of single fluorescent probe MIP@gQDs(LOD=0.25 ?mol·L^-1).There was good stability and reusability of the probe certified by 5 recycles.The established method was applied to the detection of MIT in human serum and urine,the coexisting substance,such as ions,amino acids,glucose,urea and protein in the matrix,did not interfere the detection of MIT.MIP@rQDs@SiO2 ratiometric fluorescence probe,which combined molecularly imprinted polymer with ratiometric fluorescence technique,had been applied to the selective and sensitive detection of MIT in biological fluid.?3?Preparation and characterization of organic fluorescence nanomaterial:An organic nanomaterial?DABT?with fluorescent properties was prepared in this part.DABT was prepared by simple room temperature synthesis using 3,5-diaminobenzoic acid?DABA?and 1,3,5-benzenetricarboxaldehyde?BTCO?as monomers.The composition and structure were characterized by infrared characterization,transmission electron microscopy,powder X-ray diffraction characterization,N2 adsorption-desorption analysis and thermogravimetry,and it's fluorescence spectroscopy and quantum yield were analyzed.The results showed that DABT was synthesized successfully.It not only had uniform spherical particle size distribution,good crystallinity,but also had uniform pore size distribution and good thermal stability.The absolute fluorescence quantum yield was high and fluorescence intensity was strong.These advantages make it have great application prospect in the field of analysis and detection.?4?Study of Hg²⁺ detection based on DABT:The method was established which was detected trace Hg²⁺ in water by as-prepared DABT.The quenching mechanism of DABT was further discussed.Hg²⁺ was preferentially coordinated to EDTA without coordinating with N atoms of DABT in the presence of chelating agent EDTA,so that the fluorescence of DABT was not quenched by Hg2.When EDTA was absent,Hg²⁺was coordinated with N atom of DABT,the fluorescence of DABT was quenched by Hg²⁺.Thus,DABT could detect Hg²⁺.There was a good linear relationship between analytical signal F0/F and Cng2+ when Hg²⁺ concentration at the range of 0-24.00?mol·L^-1,with the linear equation y=0.9894+0.09109x and the correlation coefficient R2=0.9932.LOD of the method was 0.030 ?mol·L^-1.The good reusability of DABT certified by 4 recycles.It was found that DABT had good selectivity to Hg²⁺,through the analysis of common metal ions in water.Thus,DABT could be used to detect the amount of Hg²⁺ in tap water and lake water.The preparation of organic fluorescent nanomaterial?DABT?was simple,rapid,and easy to use,it has great potential in fluorescent detection of other substances.