Study On Protein, Fat, Polyphenol Composition And Antioxidant Activity Of Different Soybean Varieties

Soybean is one of the important crops in China,five thousand years of cultivation history and main production areas in China's northeast,that it is a rich plant protein crop.At present,the soybean resources has aroused people's attention on development and utilization,and it gradually become the focus of research all over the world.In order to accelerate the development of the local soybean industry,studied the soybean variety selection and cultivation techniques of field experiment,the development of varieties has a better out in the fields planting of Shaanxi Yulin region in 2013.This article further research is soybean varieties in protein and amino acid,fat and fatty acids,for which is more suitable for the promotion of soybean varieties to provide the theoretical basis for further.Black beans and yellow beans contain a lot of polyphenol compounds,and has higher antioxidant activity.At present,the research of soybean polyphenols is limited to measure its total content,the composition and content of polyphenols monomer are few reported.In order to understand the soybean polyphenol composition and biological activities,developed the total polyphenol content and a monomer content is higher and the strong health effect of soybean varieties.The antioxidant activity and composition of monomer were analyzed.Hengshan black beans as raw material,through the comparison of 8 kinds of macroporous resin for Hengshan black adsorption and desorption characteristics of phenolic compound,screening of Hengshan black phenolic compounds have adsorption and desorption properties of the resin,establishing Hengshan black beans polyphenols purification process.The main research contents and results are as follows:1.This study were analyzed protein and amino acids,fats and fatty acids of 18 kinds of soybeans.The total protein and amino acid were detected by Kjeldahl analyzer and Amino acid analyzer,respectively.The fat content and fatty acid were studied by fat analyzer and Gas Chromatograph-Mass Spectrometer-computer(GC-MS).The results showed that total protein content were higher Hengshan black soybean and Shenmu lianjiatiao soybean of Yulin than others.The amino acids was highest Fendou 79 of Shanxi and the fat content of Jiyu 82 was the highest.GC-MS identified 7 kinds of fatty acids,mainly of linoleic acid and oleic acid.The unsaturated fatty acid content of 18 kinds of soybean varieties was more than 70%,of which the Zizhou black soybean,Jingbian black soybean and Jindou 19 more than 80%.2.Analysis of 18 kinds of soybean varieties of total phenol content,composition,soy isoflavones content of anthocyanins content and antioxidant capacity.Showed obvious differences between total phenol content of soybean varieties,black soybeans varieties of total polyphenols and flavonoids content is higher,lower the content of yellow soybean varieties.The anthocyanins only exist with black soybeans varieties.By DPPH free radical ability and the total antioxidant capacity FRAP study soybean polyphenols and antioxidant activity.Its antioxidant activity and total phenol content,total phenol content,the higher the higher antioxidant activity.3.There are four phenolic acids distribution forms in these soybean cultivars and they are free,esterified,glycosided and insoluble-bound phenolic acids,respectively.Showed obvious differences between total phenol content of soybean varieties,the free and esterified phenolic acids were the main phenolic acids distribution forms in all eighteen soybean samples.Chlorogenic acid and caffeic acid were the dominant phenolic acids in eight detected phenolic acids,and daidzin and genistin were the abundant isoflavones in five detected isoflavones.4.The optimal separating macroporous resin were selected through comparing static adsorption and desorption performance of eight different macroporous resins for the total phenols of Hengshan black soybean.The optimal separating parameters were as follows:the sample concentration of 1.96 mg/mL and the sampling rate of 2.14 BV/h.The contents of protocatechuic acid,daidzin and genistin were higher.The types of Hengshan black soybean polyphenols were not changed after adsorption and desorption by NKA-9 macroporous resin.NKA-9 macroporous resin purification Hengshan black soybean phenolic compounds is feasible and effective.