| The damage of skin was caused by trauma or disease,which seriously affects people's health and life.However,the normal body cannot heal large areas of skin damage and must be assisted by medical treatment.Therefore,it is urgent to seek new skin tissue repair materials to solve this problems.Electrospinning nanofibers have a wide range of application in skin tissue engineering because of their similar fiber structur as the extracellular matrix e,large specific surface area,and high porosity.In recent years,micro-patterned electrospun fibrous membranes have been received more and more attention because the microstructure of the patterned fibrous membrane can regulate its physiochemical properties and promote the adhesion,proliferation and differentiation of tissue cells.In order to solve problems such as susceptibility,insufficiency of neovascularization and slow repair on skin wound,micro-patterned organic/inorganic composite electrospun fibers were prepared by loading nano-silver particles and silicon hydroxyapatite which were on the surface of micro-patterned fibrous membranes and studied its physical,chemical and biological properties in this paper.The specific work is as follows:(1)The preparation of micro-patterned electrospun fiber membrane loaded with nano-silver particles and research on its biological properties.In view of the infection on the wound,PPG/xAgNPs composite electrospun fibrous membranes with antibacterial properties were prepared by electrospinning and in situ reduction.Firstly,Polycaprolactone(PCL),D,L-polylactic acid(PDLLA)and gelatin(Gelatin)were prepared by electrospinning to form a micropatterned electrospun fiber membrane(PPG)with a round hole morphology which AgNPs could pass through.Then,AgNPs was loaded on the surface of the PPG fiber with reduction method.The experimental results showed that AgNPs were uniformly loaded on the surface of the fiber and their size was between 20-60 nm.The vitro results showed that when the loading of AgNPs was 0.01%(PPG/0.01AgNPs),the composite fiber membrane had an inhibitory effect on E.coli and could promote the proliferation and adhesion of endothelial cells(HUVECs).Hence,PPG/0.01 AgNPs patterned electrospun fibrous membranes have potential application on infected wounds.(2)The micro-patterned electrospun fibrous membranes and surface silicon substituted hydroxyapatite to promote acute wound healing was studied.To solve the problem of slow wound healing in diabetes,PP/xSi-HAp composite fiber membrane was prepared by combining electrospinning and pulsed laser deposition(PLD).Firstly PCL,PDLLA were used as raw materials.The micro-patterned fibrous membranes were prepared by electrospinning.Then the PP/x Si-HAp fibrous membranes with multi-level structural and surface silicon substituted hydroxyapatite was prepared by PLD.The results showed that x Si-HAp was successfully deposited on the surface of nanofibrous membranes after laser ablation.the size of xSi-HAp was between 10-30 nm,and the hydrophilic properties of composite fibrous membranes.In vitro study showed that the composite fiber membrane can significantly promote the proliferation,migration and angiogenesis of HUVECs.In vivo study further showed that PP/2.4Si-HAp composite fiber membrane can promote angiogenesis,collagen deposition and angiogenesis-related gene expression,which accelerate wound healing process in diabetic mice.Therefore,the double-oriented structure of the composite fibrous membrane and xSi-HAp components can promote the healing of diabetic wounds,which is potential for application in the chronic wound repair.In summary,we designed a micro-patterned with organic/inorganic composite fibrous membrane and studied its antibacterial and angiogenic effects in skin wound repair.The micro-patterned structure of the composite fiber membrane and the inorganic particles components cooperate to promote the repair of the wound surface,which provide a new method for designing a novel biological material. |
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