| Electrospinning technology is a new material fabrication technology to fabricating nanofiber membrane,which has broad application fields in food,agriculture and biomedicine.In recent years,electrospinning technology developed rapidly in the food science,and food materials with the advantages of safety,diverse structure and low cost,greatly promotes the development of electrospinning technology.Using food raw materials to prepare nanofiber membranes and provide efficient delivery vehicles for food functional components has become the main development direction of electrospinning technology,and wound dressing is one of the research hotspots.Sesamol is a polyphenolic food functional component in sesame,having high application value in the fields of anti-inflammatory,anti-oxidation and anti-aging.Recently,the novel function about accelerating wound healing of sesamol had also attracted much attention.In this study,two widely used food raw materials,cellulose acetate(CA)and zein,were used to fabricate nanofiber membranes loaded with sesamol,with the purpose to study the effect of composite nanofiber membranes on wound healing acceleration and speculate the molecular mechanism of action.The main research contents are as follows:(1)The blended nanofibers were prepared by CA and zein,the spinning effects of different concentrations and proportion of polymers were studied.The results showed that the critical concentration of polymers was between 15% and 25%(w/v),the concentration of stabilizing fibers was 22.5%.Morphology,structure and properties of fibers were related with the ratio of CA to zein.When the ratio of CA/zein was 12:8,the fiber obtained the low diameter and distribution,the stable intermolecular structure,the strong interaction,the high thermal stability and hydrophobicity,in addition to the low infiltration speed and high stability in water.The concentration of zein less than 5% destroyed the intermolecular forces of CA,increased diameter distribution,reduced fibers stability,thermal stability and stability in water.A content of 10% of zein caused the failure of electrospinning because of the poor fiber structure.(2)The sesamol-CA/Zein composite nanofiber membrane was prepared by loading sesamol on CA/zein blended nanofiber membrane.It was found that the effect of sesamol on fiber morphology was limited,mainly relying on methylenedioxy group to interact with CA and zein,but the intermolecular force was weak.Melting point and thermal stability of sesamol was enhanced by fiber structure.However,reduced thermal stability and increased hydrophilicity of the fiber were observed due to the presence of sesamol.The distribution of sesamol in the fiber was uneven,surface and superficial layer had the higher concentration,which led to a “burst release period” of sesamol in the initial stage of release.(3)Diabetic mouse model was established by intraperitoneal injection of streptozocin(STZ),and a full-thickness skin wound with a diameter of 5 mm was created on the back of mice to study the effect of sesamol-CA/Zein composite nanofiber membrane on the wound healing process.The results showed that high-dose sesamol group(5% of total polymer concentration,w/w)promoted formation of myofibroblasts by enhancing TGF-? signaling pathway transduction,and promoted keratinocyte growth by inhibiting chronic inflammation in wounds,thus enhancing wound healing in diabetic mice.In summary,sesamol-CA/Zein composite nanofiber membrane was fabricated successfully,which exhibited the effect of accelerating wound healing in diabetic mice.This study could further broaden the application range of sesamol,CA and zein,and provide reference for the design and development of new wound dressings in the future. |
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