Construction Of Antioxidant Hemodialysis Membrane And Its Biocompatibility Study

Polysulfone(PSF)has been widely used in the field of artificial blood vessel and tissue engineering because of its excellent thermal stability,chemical stability and its own non-toxic.However,the isopropyl group on polysulfone is easy to produce methyl radicals when it comes in contact with strong oxidants,which aggravates oxidative stress in patients with uremia and limits its application in the field of blood contact materials.In this work,we introduced the modifier to improve the membranes of anti-oxidation resistance and biocompatibility.Silybin(SLB)is a natural anti-oxidant.Using PSF as membrane material and SLB as modifier,anti-oxidation PSF/SLB hemodialysis membrane was prepared by immersion precipitation phase inversion method.The surface composition and morphology of modified membranes were characterized by X-ray photoelectron spectroscopy(XPS)and field emission scanning electron microscope(FESEM),and results showed that functional monomers introduced into the membranes migrated and enriched to the membranes surface during membranes formation process.With the increase of the content of SLB in casting solution,the pure water flux of PSF/SLB blend membrane increases,the hydrophilicity increases.The 1,1-diphenyl-2-picrylhy-drazyl(DPPH)and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)maximum scavenging rate of PSF/SLB blend membrane compared with the pure membrane was significantly different.Compared with the pure PSF membrane,the SPC/PSF blend membrane not only has less platelet and red blood cell adhesion,but also has a stronger ability to inhibit the production of superoxide dismutase(SOD)in whole blood.Silybin-Phosphatidylcholine Complex(SPC)has good oxidation resistance and biocompatibility.The PSF/SPC hemodialysis membrane with antioxidant activity was made using PSF and SPC as membrane material by liquid-liquid phase separation method.SPC was segregated to PSF/SPC blend membrane surface as confirmed by XPS.For the PSF/SPC blend membrane,the pure water flux increased to 187.9±6.9L/(m2h)and hydrophilicity increased and the bovine serum albumin(BSA)rejection rate decreased slightly compared with the pure PSF membrane.With the increase of the content of SPC on the membrane surface,the ability of scavenging DPPH and ABTS free radical were increased to 88.3±1.3%and 99.4±0.3%.For the PSF/SPC blend membrane,the stability of free radical scavenging capability under typical hemodialysis condition was good.Compared with pure PSF membrane,the PSF/SPC blend membrane had good cell compatibility.Compared with the pure PSF membrane,the platelet adhesion of PSF/SPC blend membrane was decreased,and the blood compatibility was improved.The PSF/SPC blend membrane had strong ability to inhibit superoxide dismutase(SOD),while pure PSF membrane stimulated the production of SOD.The results indicated that blend modification by SPC and PSF provided practical application of the membranes with good antioxidant properties;and a new type of PSF/SPC hemodialysis membrane was expected to be applied in the field of hemodialysis and alleviate the oxidative stress status of the patients.