Controllable Preparation And Dual Drug-loading Properties Of Emulsion Electrospun Micro-nano Fiber Membranes

In recent years,with the rapid development of society and people living standard rise,people has higher requirement for the quality of life,but in order to solve the frequent dosing of the pain and inconvenience to people,researchers have been trying to find a safe and effective drug slow delivery system.In this topic,we select good biocompatibility,poly(lactic acid)(PLA)as well material,select the representative anti-cancer drugs camptothecin(CPT),astragalus polysaccharide(APS)and doxorubicin(DOX)as template drug.Prepared by the emulsion electrospun nanofibers successful get slow controlled release drug and anti-cancer fiber membrane.This subject adopts instead turn method,polylactic acid as base material,acetone,N,N dimethyl formamide as solvent,gelatin as a stabilizer,SMA,Span80 as emulsifier,successful preparation of a stable oil-in-water(O/W)emulsion.The PLA/PEG micro/nano-fibers membrane with different fiber morphology was prepared by using polyethylene glycol of different molecular weight to control the emulsion.Using FTIR,XRD,XPS,SEM,TEM and other analytical methods to characterize the structure and morphology of the structures,the spinning conditions of the preparation of the PLA/PEG micro/nano-fibers membrane were determined by SEM observation.In this condition,the carrier of different fiber morphology was prepared with the PLA/PEG micro/nano-fibers membrane film,whose hydrophilic properties were good,the composite membrane appearance was different,and the hydrophilicity was different.The MTT method was used to test the cytotoxicity of the PLA/PEG micro/nano-fibers membrane prepared by mouse fibroblasts(L929 cells).In.vitro drug release,the test results showed that it was free of cytotoxicity and good biocompatibility and the drug use is high,up to 29.5%and the drug utilization rate is high,up to 95%.The drug release has a slow release effect on drug release,and the release rate is different in different pH phosphate buffer solution.At the same time,the two drugs have been Fick diffusion as the leading factor.Use of drugs with red fluorescence DOX as hydrophilic drug model,electrostatic spinning,and the cover glass to receive a certain amount of samples,and then observed by laser confocal microscope(CLSM)DOX in drug distribution of PEG/PLA micro/nano-fibers membrane.In the appearance of "string-in-beads",the hydrophilic drug DOX mainly covered the surface of beads;In the morphology of "nano-fibers",DOX is mainly distributed in nanoscale fibers.