Cloning Of The Beet M14 Floral Organ Expression Gene

The monosomic addition lines of Beta corolliflora in sugar beet M14(2n=19) was produced by Professor Dedong Guo by interspecific cross of cultivated Bet vulgaris with wild species Beta colloroflora. It is an aneuploid hybrid containing both sexual and apomictic traits, hi order to provide molecular basis for revealing the genetic mechanisms of M14, the purpose of this work is to amplify complete cDNA sequences by using LA-PCR and rapid amplified cDNA of ends (RACE) techniques, based on transcript-derived fragments (TDFs), which are previously isolated in our laboratory.Our results show mat four complete cDNA sequences, called here TFG5-2, TG75, T8F5-3, T7F1-1, have been cloned. They have the open reading frames of1374bp,1032bp,1464bp and1665bp encoding four proteins of457,343,487and554amino acids, respectively. The blastn search of the entire GenBank collection shows that the TFG5-2has73%sequence homology with that of Jatropha curcas L, the function of the protein encoded by TFG5-2, however, remains unknown. The cDNA sequences TG75, T8F5-3and T7F1-1have84%,81%and98%sequence homology with those of ubiquitin,5-methyltetrahydrofolate and S-adenosyl-L-homocysteine hydrolase, respectively, In addition, the physical and chemical characteristics of the above-mentioned four proteins have also been discussed. Our results in this work have clearly demonstrated that we have successfully amplified four full-length cDNAs.