A Proteomic Study Of Pyrimycin Regulating The Immune System Of Rice

Southern rice black-streaked dwarf virus(SRBSDV), a new rice dwarf disease which was firstly discovered and named in China, is a member of the genus Fijivirus, family Reoviridae. SRBSDV was outbreak in many provinces in southern China in recent years and it caused severe damage on rice production.Cytosinpeptidemycin, a new antiviral agent, was widely used to control of SRBSDV. Field trials indicated that Cytosinpeptidemycin demonstrated appreciable control efficiency against SRBSDV. However, the action mechanism of Cytosinpeptidemycin in protecting rice from SRBSDV infection is remain unclear. Therefore, to clear the key target protein and pathway plays an important role in creation of new pesticide.In this study, the changes of the enzymatic activities of POD, SOD and CAT in rice tissues, treated with Cytosinpeptidemycin, were determined using ultraviolet spectroscopy and the results preliminary demonstrated that Cytosinpeptidemycin could enhance the activities of POD, SOD, CAT enzymes. Meanwhile, qRT-PCR analysis showed that Cytosinpeptidemycin could improve the relative expression of Ascorbate POD?Glutathione POD?Chitinase, and PR1 genes. In addition, we compared the differentness of proteomics of Cytosinpeptidemycin responses in SRBSDV-infected rice using label-free proteomics technology and results showed that 320 up-regulated and 155 down-regulated proteins were differentially expressed in Cytosinpeptidemycin treatment group. Especially, among these differentially accumulated proteins, we found that Calmodulin-like(CML) protein, which played a key role in salicylic acid signaling pathway, was specific up-regulated expression and qRT-PCR analysis showed that Cytosinpeptidemycin could improve the relative expression of ICS1?PAD4?PAL?EDS1, and NPR1 genes.