Study On Quality Characteristics And Commodity Specification Grade Of Qianghuo

Notopterygii Rhizoma et Radix, originates from the dried rhizome and root of Notopterygium incisum Ting ex H. T. Chang and Notopterygium franchetii H. de Boiss. (family Umbelliferae),only contained in the Sheng Nong's herbalclassic,Under listed in Angelicae Pubescentis Radix as an alias, it is famous for itsdiaphoretic, antifebrile, antirheumatic and anodyne characteristics in the treatment of rheumatism, headaches and colds. Notopterygii Rhizoma et Radix, is mainly distributed in Ganzi and Aba County Sichuan Province, Qinghai Province and Gansu Province of China. Seventy-six kinds of medicinal products specifications in 1984. writes"it is called Chuan-qiang in Chinese of primary producing in Sichuan Province, especially in Aba and Ganzi; Xi-qiang called in Chinese is primary produced in Qinghai and Gansu Province. According to the character and quality of different specifications. Chuan-qiang is divided into Silkworm Notopterygium (first class) and Striped Notopterygium (second class), Xi-qiang is departed into Silkworm Notopterygium (first class), Irregular-nodal Notopterygium (second class) and Striped Notopterygium(third class). This standard continuously uses today, with the continuous changes in the Chinese herbal medicine commodity market, this standard has been unable to meet the needs of Chinese herbal medicine market. Market sales of Notopterygii Rhizoma et Radix commodity specifications confusion, specifications and classification of no strict promulgated in accordance with national standards to perform, which also caused the market price of notopterygium confusion, the market sales of Notopterygii Rhizoma et Radix commodity specifications uneven, useful Angelicae Pubescentis Radix, Heracleum hemsleyanum Michaux doped pseudo phenomenon. At the same time, with continuous improvement of notopterygium planted in technology, market notopterygium except for wild, also appeared in the sales of small amounts of notopterygium planted, and cultivation of Notopterygii Rhizoma et Radix commercial specification is more a lack of basic research and market promotion.This research is in order to distinguish between notopterygium specifications based in the traditional experience differential, within in quality as the basis, establish the correlation between traits and herbal quality, in order to achieve Notopterygii Rhizoma et Radix high quality, establish a set to meet the market need of notoptery-giummedicinal commercial specification.The results are as follows:1. The property characteristics of 41 batches of samples of notopterygium medicinal were identified.Using a number of traditional Chinese medicine experts in accordance with the appearance of different identified the notopterygium distinguish between different specifications. which is divided into Silkworm Notopterygium, Striped Notoptery-gium, Bamboo Notopterygium, Residual Notopterygium, lump Notopterygium, Irregular-nodal Notopterygium and Cultivated Notopterygium. Experiment from medicine area, shape, color, size, cross section, gas and taste, etc, to study and adopt the method of frequency statistics and color recognition of chromatic meter, compre-hensive result analysis:Silkworm Notopterygium, often called Can-Qiang in Chinese, are part of rhizomes, formed by densely thicknodes, with very short internedes, and look like silkworm Notopterygium; Striped Notopterygium, often called Tiao-Qiang in Chinese, are formed by obscure nodes bearing roots, strip shape; Bamboo Notopterygium. often called Zhu-Jie-Qiang in Chinese, are formed by long and thin internodes and thick nodes, and look like bamboo stems:Residual Notopterygium. often called Wei-Qiang in Chinese, is by broken rhizome and radix form; Lump Notopterygium. often called Ge-Da-Tou in Chinese, look like irregular clumps; Irregular-nodal Notopterygium, often called Da-Tou-Qiang in Chinese, are part of rhizomes, formed by several pieces of rhizome parts with dense nodes join together to form an irregular compact mass; Cultivated Notopterygium, often called Zai-Pei-Qiang in Chinese, the number of root clusters in rhizome, long strip.2. For different specifications of Notopterygii Rhizoma et Radix were identified by TLC.In nodakenin as reference, the specifications and reference substance in the corresponding position was the same color spots, each TLC consistent, but each spot color shades are different in wild notopterygium, Shows that it has the same chemical composition, but there are differences on content, cultivated Notopterygium and wild notopterygium of TLC difference is bigger, but there are differences on the content.3. Analysis of volatile oil by GC-MSEach product specifications Notopterygium common components contained 34 species, relatively content is above 1% are nine kinds. Silkworm Notopterygium, the relative content of each component is higher, followed by lump Notopterygium, the lowest is residual Notopterygium; Notopterygium forbesii common components contained 36 species, relatively content is above 1% are thirteen kinds, higher relative content is Bamboo Notopterygium. Silkworm Notopterygium and Irregular-nodal Notopterygium similar content; Notopterygii Rhizoma et Radix and Notopterygium forbesii in the volatile oil chemical composition little difference, but each component relative contents were larger, alpha pinene. ?-pinene, limonene etc component in Notopterygii Rhizoma et Radix was significantly higher than that of Notopterygium forbesii,2-borneol, sinistral bornyl acetate, (+)-flower thujene into Notopterygium forbesii was significantly higher than that of Notopterygii Rhizoma et Radix. Therefore, using GC-MS as the essential oil component analysis can be well identified Notopterygium forbesii and Notopterygii Rhizoma et Radix and also can distinguish between the same source notopterygium different specifications.4. HPLC fingerprint for the identification of various specifications Notopterygii Rhizoma et Radix.The commodity specifications notopterygium under the same elution condition peak total similarity is above 0.8, and similarity is higher, which can not use specific chromatogram to distinguish; The same specifications of the different sources of notopterygium similarity difference is obvious, therefore, can use specific chromatogram to distinguish; similarity of cultivated Notopterygium is low, less common peak,with specific chromatogram of various specifications wild notopterygium difference is more apparent.5. For various specifications notopterygium were moisture, total ash and acid insoluble ash of content examination.The result shows that all the goods specification notopterygium of moisture was 9%?15%, and different regions with specification notopterygium moisture difference is greater, may be associated with processing way; Most of the total ash content lower than 8.0% of different specifications Notopterygium, acid insoleble ash content lower than 3.0%, relatively high of cultivation notopterygium and Irregular-nodal Notopterygium about total ash and acid insoluble ash content may be due to its high sand more impurities.6. For each commodity specification notopterygium alcohol soluble extract content determination.It is found that different specifications notopterygium alcohol soluble extracts differences, Silkworm Notopterygium and cultivated Notopterygium content are higher, Residual Notopterygium content is the lowest, the rest of the goods specification extract content difference was not significant.7. The content determination of volatile oil of the commodity specifications Noto-pterygium.It is found that the highest content of volatile oil is Silkworm Notopterygium about 2.02%, the lowest content is Striped Notopterygium about 1.48%, and Lump Notopterygium of volatile oil content is significantly higher than Bamboo Notopterygium, Residual Notopterygium, Irregular-nodal Notopterygium and Cultivated Notopterygium, no significant differences between BambooNotopterygium, Residual Notopterygium. Irregular-nodal Notopterygium and Cultivated Notopterygium.8. Detect the main component of notopterol and isoimperatorin of the specifications of notopterygium by HPLC.It is found thatthe highest total content is Silkworm Notopterygium about 1.32%, the lowest content is Striped Notopterygium about 0.62%, the rest of the goods size difference is not obvious.9. The same place of origin of different specifications of Notopterygii Rhizoma et Radix about pharmacodynamics of anti-inflammatory and analgesic comparative study.The results showed that the anti-inflammatory effect of Silkworm Notopterygium and Lump Notopterygium is the most significant, followed by the Bamboo Notopterygium, there was no significant effect of are Cultivated Notopterygium, Striped Notopterygium and Residual Notopterygium. Analgesic effect of the most obvious is the Silkworm Notopterygium and Lump Notopterygium, followed byBamboo Notopterygium and Cultivated Notopterygium. Residual Notopterygium and Striped Notopterygium are no obvious effect.Integrated the experimental results, it is found that Notopterygii Rhizoma et Radix specifications can be classified according to the sources and characters, the research group considered practical convenience, the selected traits into basis, by weighting method of various specifications notopterygium statistics, the quality of various specifications notopterygium sequence is:Silkworm Notopterygium> Irregular-nodal Notopterygium> BambooNotopterygium? Residual Notopterygium>Striped Notopterygium.Cultivated Notopterygium is equal to Irregular-nodal Notopterygium.