The Mechanism Of Intervention Of The Dopamine Adenylate Cyclase Pathway In The Hypothalamic Rats Of HPRL Model Rats With Liver And Spleen As The Core

ObjectiveThe purpose of this paper was to investigate the set up animal model of HPRL by Pituitary transplantation and to select the optimal D1, D2 receptor of the hypothalamus when animal model of HPRL were set up successful,meanwhile, to observe the influence of Adenosine cyclization dopamine pathways in the hypothalamus of HPRL animal model treating with Jiawei Xiaoyao powder as the core of Liver and Spleen.Methods1.We divided SD rats randomly into blank control group,model group in 14days, model group in 18days and model group in 21days.Then to detect prolactin level by using enzyme-linked immunoassay and observe follicle changes by using HE staining between each group of rats.2.We divided SD rats randomly into blank control group,model group,SCH23390-1 group and SCH23390-2 group to select dopamine Dl receptor antagonist of the hypothalamus through prolactin level of rats serum and expression quantity of Dl receptors in the hypothalamus by using enzyme-linked immunoassay and Immunohistochemical method.We divided SD rats randomly into blank control group?model group,sulpiride-1 group,sulpiride -2 group,sulpiride -3 group and haloperidol group to select dopamine D2 receptor antagonist of the hypothalamus through prolactin level of rats'serum and expression quantity of D2 receptors in the hypothalamus by using enzyme-linked immunoassay and Immunohistochemical method.3.To observe the influence of Adenosine cyclization dopamine pathways in the hypothalamus of HPRL animal model treating with Jiawei Xiaoyao powder as the core of Liver and Spleen,we divided SD rats randomly into blank control group,model group,bromocriptine group,Chinese medicine high dose group,Chinese medicine middle dose group,Chinese medicine low dose group,haloperidol group,haloperidol with Chinese medicine middle dose group.haloperidol with bromocriptine group,SCH23390 group,SCH23390 with Chinese medicine middle dose group and SCH23390 with bromocriptine group.Then we take the serum and hypothalamus after 30days and test prolactin level of rats'serum and content of AC and cAMP in the hypothalamus of each group by using enzyme-linked immunoassay,determine the relative expression quantity of DIR?D2R?GS and Gi gene in the hypothalamus of each group by using RT-PCR method.Results1.It was suggested that serum PRL levels of HPRL animal model maded with pituitary transplantation would be higher than blank control group, more than25 ng·mL-1.At the same time,the number of bilateral growth of poor ovarian follicle of model rats increased significantly(P< 0.05),the number of mature follicle decreased significantly (P< 0.05),this change in modeling the 18th day is particularly acute.2.SCH23390-1 group and SCH23390-2 group both can cause the loss of PRL of model rats and depletion in numbers of D1R(P<0.01),and SCH23390-2 group be superior to SCH23390-1 group in antagonism action of D1R(P<0.01).Sulpiride each dose group and haloperidol-1 group all can cause the rise of PRL of model rats and increasing in numbers of D2R(P<0.01),and haloperidol-1 group be superior to Sulpiride each dose group in antagonism action of D2R.3.How to observe the influence of Adenosine cyclization dopamine pathways in the hypothalamus of HPRL animal model treating with Jiawei Xiaoyao powder as the core of Liver and Spleen was as followed:the serum PRL level increased, the relative expression quantity of D1R?GS mRNA increased significantly (P<0.01)and D2R?Gi mRNA decreased significantly (P<0.01)in hypothalamus tissues,the content of AC and cAMP increased significantly (P<0.01) in the cell of the hypothalamus organization,which was in model control group compared with blank control group.And compared with model control group,bromocriptine group and Chinese medicine each dose group all cause the serum PRL level decreased, the relative expression quantity of D1R?GS mRNA decreased significantly (P<0.01)and D2R-. Gi mRNA increased significantly (P<0.01)in hypothalamus tissues,the content of AC and cAMP decreased significantly(P<0.01) in the cell of the hypothalamus organization.The influence of D1R,GS mRNA,AC,cAMP of Adenosine cyclization dopamine pathways in the hypothalamus of HPRL animal model of D2R antagonists(haloperidol group) was the same as model control group and surpass the model control group.Haloperidol with Chinese medicine group and haloperidol with bromocriptine group can reduce the influence of the group,compare with bromocriptine,the Chinese Medicine stronger on the influence of Adenosine cyclization dopamine pathways in the hypothalamus of D1R.;The influence of D2R,Gi mRNA,AC,cAMP of Adenosine cyclization dopamine pathways in the hypothalamus of HPRL animal model of D1R antagonists(SCH23390 group) was on the contrary to model control group,SCH23390 with Chinese medicine group and SCH23390 with bromocriptine group can reverse the influence of the group to some different extent.compare with Chinese Medicine,the bromocriptine stronger on the influence of Adenosine cyclization dopamine pathways in the hypothalamus of D2R.Conclusion1.It is concluded that the best animal model of HPRL through pituitary transplantation was model building for 18 days.2.we choose SCH23390 1mg·kg-1 as D1R antagonist.we choose Haloperidol lmg·kg-1 as D2R antagonist.3.Jiawei Xiaoyao Powder can cure HPRL,and there is a concentration-response relationship to a certain extent.The influence of Adenosine cyclization dopamine pathways in the hypothalamus of HPRL animal model of Jiawei Xiaoyao Powder:in order to achieve the purpose of the treatment of hyperprolactinemia through exciting D2R and inhibiting D1R to controling serum PRL level.