| Objectives: To detect the distribution of miR-126 rs4636297G/A polymorphism in Guangxi healthy population,and compared with other different regions of the crowd may help us understand the distribution features of miR-126 rs4636297G/A polymorphism in Guangxi population.Methods: The single-base extended polymerase chain reaction(Snapshot PCR)genotyping assay and DNA sequencing were used to detect the distribution of miR-126rs4636297G/A in Guangxi 456 healthy population.The SNPs data of Beijing,European,Japanese and African population were obtained from the Pub Med(SNP)database.The SNP datas were compared inthe five populations to analyze the similarities and differences in thedistribution of polymorphic rs4636297G/A in these five populations.Results:We found that there existed miR-126 rs4636297G/A gene polymorphism in Guangxi populations.Both of the polymorphisms have three genotypes.The distribution of miR-126 rs4636297G/A gene polymorphism in Guangxi populations was in HWE(P>0.05).Comparing with other four populations,the genotype and allelic frequencies of miR-126rs4636297G/A polymorphism had significant difference between Guangxi and CEU,and YRI populations(both P<0.05);however,the genotype and allelic frequencies of miR-126 gene rs4636297G/A polymorphism had no significant difference between Guangxi and HCB,and JPT populations(both P>0.05).Conclusion: There existed miR-126 rs4636297G/Apolymorphism in Guangxi healthy population.The distribution of miR-126 rs4636297G/A polymorphism in Guangxi populations was in HWE(both P>0.05).The genotype and allelic frequencies of miR-126 rs4636297G/A polymorphism was significantly different in different populations.These differences might account for the different morbidity of miR-126 rs4636297G/A related disease among different populations.Objective:Investigating the relationship between miR-126 gene polymorphism and ischemic stroke susceptibility.To investigate the expressionof miR-126 inischemic stroke patientsand normal controls and analyze theassociation between the miR-126rs4636297G/A polymorphismand the expression of miR-126.Methods: The single-base extended polymerase chain reaction(Snapshot PCR)genotyping assay and DNA sequencing were used to detect the distribution of miR-126rs4636297G/Apolymorphism in 456 Guangxihealthy controls and 592 ischemic stroke patients.The genetic susceptibility of miR-126 rs4636297G/A polymorphism to ischemic stroke was calculated by statistical software.The expression level of miR-126 in case and control group was detected by SYBR Green real-time quantitative PCR and the correlation between miR-126 gene rs4636297G/A polymorphism and miR-126 expression was analyzed.Results:We found that there existed miR-126 rs4636297G/A gene polymorphism both in case and control populations.All of the polymorphisms have three genotypes.The distribution of miR-126 rs4636297G/A gene polymorphism in case and control group were in HWE(both P>0.05).In the present study,we found that the distribution of miR-126 rs4636297G/A polymorphism in case and control group showed significant difference(P<0.05).Compared with the normal controls,the GA(33.8%vs.24.8 %)and AA(5.9%vs.2.5%)genotypes were significantly decreased in ischemic stroke patients;GA and AA genotypes were associated with significantly decreased risk of ischemic stroke(GA vs.GG: OR=0.61,95%CI=0.47-0.80,P<0.001;AA vs.GG: OR=0.36,95%CI=0.19-0.68,P=0.001).In the genetic model analysis,we found that both dominant model and recessive model of miR-126 rs4636297G/A polymorphism were associated with decreased ischemic stroke risk(dominant model,AA+GA vs.GG:OR=0.57,95%CI= 0.44-0.74,P=<0.001;recessive model,AA vs.GA+AA:OR=0.41,95%CI= 0.22-0.79,P=0.006).By logistic regression analysis,the association also existed(GA vs.GG: AOR=0.64,95%CI=0.44-0.95,P=0.025;AA vs.GG: AOR=0.32,95%CI=0.14-0.74,P=0.007;dominant model,AA+GA vs.GG:AOR=0.58,95%CI= 0.40-0.84,P=0.004;recessive model,AA vs.GA+AA:AOR=0.37,95%CI= 0.16-0.82,P=0.015).Compared with normal controls,serum miR-126 level was significantly decreased in IS patients(IS vs.control = 6.57±1.50 vs.9.86±1.92,P<0.001).Notably,patients carrying the rs4636297 GA/AA genotype had a relative higher level of miR-126(GG vs.GA / AA = 6.08±1.47 vs.7.53±2.00,P<0.001),but the association did not existed in the control group(GG vs.GA / AA = 9.77±1.67 vs.10.6±1.19,P = 0.485)Conclusion: The GA/AA genotypes of rs4636297G/A polymorphism was negatively correlated with ischemic stroke risk.MiR-126 rs4636297G/A might be a protective factor for the occurrence of ischemic stroke.In addition,the abnormal reduction of miR-126 in stroke patients may be a potential biomarker or therapeutic target for ischemic stroke.Objectives: To detect the distribution of miR-21 rs1292037T/C polymorphism in Guangxi healthy population,and compared with other different regions of the crowd may help us understand the distribution features of miR-21 rs1292037T/C polymorphism in Guangxi population,and lay the genetic foundation for the follow association study.Subjects and Methods: The single-base extended polymerase chain reaction(Snapshot PCR)genotyping assay and DNA sequencing were used to detect the distribution of the miR-21rs1292037T/C in healthy population.The SNPs data of Beijing,European,Japanese and African population were obtained from the Pub Med(SNP)database.The SNP datas were compared in the five populations to analyze the similarities and differences in the distribution of polymorphic miR-21 rs1292037T/C in these five populations.Results:We found that there existed miR-21 rs1292037T/C gene polymorphisms in Guangxi healthy populations.The polymorphism has three genotypes.The distribution of miR-21 rs1292037T/C polymorphism in Guangxi populations was in HWE(P>0.05).Comparing with other four populations,the genotype and allelic frequencies of miR-21 gene rs1292037A/G polymorphism had significant difference between Guangxi and CEU,YRI and JPT populations(both P<0.05);however,the genotype and allelic frequencies of miR-21 gene rs1292037A/G polymorphism had no significant difference between Guangxi and HCB populations(both P>0.05).Conclusion: There existed miR-21 rs1292037T/C gene polymorphism in Guangxi population.The distribution of miR-21 rs1292037T/C gene polymorphism in Guangxi population was in HWE(P>0.05).The genotype and allelic frequencies of miR-21 rs1292037T/Cpolymorphism was significantly different in different populations.These differences might account for the different morbidity of miR-21 rs1292037T/C related disease among ethnics.Objective:Investigating the relationship between miR-21 rs1292037T/C polymorphism and ischemic stroke susceptibility.To investigate the expression of miR-21 in ischemic stroke patients and normal controlsandanalyze the association betweenmiR-21 rs1292037T/C polymorphismand the expression of miR-21.Methods: The single-base extended polymerase chain reaction(Snapshot PCR)genotyping assay and DNA sequencing were used to detect the distribution of the miR-21rs1292037T/C,in Guangxi 456 healthy controls and 592 ischemic stroke patients.The genetic susceptibility of miR-21 rs1292037T/C polymorphism to ischemic stroke was calculated by statistical software.The expression level of miR-21 in case and control group was detected by SYBR Green real-time quantitative PCR and the correlation between miR-21 gene rs1292037T/C polymorphism and miR-21 expression was analyzed.Results:We found that there were miR-21 rs1292037T/C polymorphisms both in case and control populations.All of the polymorphisms have three genotypes.The distribution of miR-21 rs1292037T/C polymorphisms in case and control group were in HWE(both P>0.05).In the present study,we found that the distribution of miR-21 rs1292037T/C polymorphism in case and control group showed no significant difference(P>0.05).In the genetic model analysis,we found that both dominant model and recessive model of miR-21 rs1292037T/C polymorphism were not associated with ischemic stroke risk(dominant model,CC+CT vs.TT:OR=1.22,95%CI= 0.94-1.59,P=0.137;recessive model,CC vs.CT+TT:OR=1.24,95%CI= 0.90-1.71,P=0.191).By logistic regression analysis,the association also did not existed(CT vs.TT: AOR=1.21,95%CI=0.81-1.80,P=0.349;CC vs.TT: AOR=1.47,95%CI=0.86-2.50,P=0.159;dominant model,CC+CT vs.TT:OR=1.28,95%CI= 0.88-1.86,P=0.204;recessive model,CC vs.CT+TT :OR=1.37,95%CI= 0.85-2.21,P=0.192).Compared with normal controls,serum miR-21 level was significantly increased in IS patients(IS vs.control = 34.65±7.26 vs.26.89±6.81,P<0.001).The miR-21 levels were not associated with rs1292037T/C polymorphism.Conclusion: MiR-21 rs1292037T/C polymorphism was not associated with ischemic stroke risk.The abnormal increseaed miR-21 in stroke patients may be a potential biomarker or therapeutic target for ischemic stroke. |
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