Baiziren Ingredients And Preparation Research

Purpose: This study aims to traditional Chinese medicine theory,under the guidance of reference literature study of platycladi seed composition.Respectively from platycladi seed component separation and identification to determine platycladi seed,the known and unknown components of terpenoids composition for the determination of extraction technology and optimize preparation process,clear pharmacological action,determine the platycladi seed protein content in different regions,the level of clinical pharmacology of platycladi seed research provides the basis.Material and methods:1.Scraper using column chromatography,thin layer chromatography method,starting with cyclohexane as solvent,the squeezing of platycladi seed oil separation,with cyclohexane:ethyl acetate 100:1,99:1,98:1...90:1...80:1...10:1...1:1 proportion of platycladi seed oil such as column chromatography,with thin slabs fractions classification recycling,and then to scraper purification.Using nuclear magnetic resonance hydrogen spectrum and carbon compounds were isolated from preliminarily determined,by mass spectrometry to determine the compound structure.2.Using ultrasound,reflux and microwave and homogeneous method,using single factor experiment investigation dripping speed,coolant and cooling factors such as temperature,liquid ratio of the homogeneous time,temperature,material and the number of four factors,three levels of each factor,using L9(34)orthogonal experiment the optimization extraction process.3.Using coomassie brilliant blue method for determination of platycladi seed protein content in different regions.4.Observation of platycladi seed the effects of total terpenoid components on spontaneous activity of mice,the sodium pentobarbital sleeping time in mice induced and the effects of the number of sleep and the survival time of hypoxia mice.5.Using polyethylene glycol(peg)and alvin Nick as a carrier material,melting of platycladi seed prepared by terpenoids dropping pill.With pills weight difference,roundness and dissolution as indexes,the single factor experiment investigation dripping speed,coolant temperature and cooling etc.;By orthogonal experiment optimizing proportion of polyethylene glycol(peg)and alvin Nick,drugs and matrix formula,solution temperature,dripping cheng preparation process conditions.Result:1 Cyclohexane: ethyl acetate 100:1 to cyclohexane: ratio of ethyl acetate to 1:1 silica gel column chromatography,and then to prepare thin layer chromatography scraper was Y1-Y9 nine compounds.Based on the physicochemical properties and 1H NMR,13 C NMR,Y1-Y9 was identified as a ceramide compound.2 Determine the methanol solution as the extraction solvent,the solid-liquid ratio of 1:10,at80 degrees Celsius,homogeneous 10 min,homogeneous extraction for the 1 time for the best extraction process.3 The origin of protein in different areas of Platycladi seed is about 0.2-1.6mg/g.4 Platycladi seed total terpenoids(in large doses)can inhibit the spontaneous activity of mice,prolong the mice sleep time induced by sodium,and increase the number of sleeping mice,and increase the survival time of hypoxia mice.5 The best preparation conditions,matrix composition PEG: Polanyi J for 5:1,ratio of drug and matrix 1:2,dropping liquid when the temperature is 80 degrees centigrade,drop 10 cm,drop 40 drops of /min,liquid wax as coolant,cooling temperature gradient(50 to 10 DEG C).Conclusion:1 Y1-Y5 polar size is similar to the silica gel column chromatography when the proportion of cyclohexane: ethyl acetate need to be carefully divided in order to get Y1-Y9.9 compounds are ceramide compounds.2 The operation is simpler than other conventional homogeneous extraction extraction method, eliminating the need for drying of material,crushing steps,and the extraction of material crushing and effective components simultaneously,is a kind of extraction of the effective components of plant fast method of high strength.3 The established protein contains a stable and reliable method.To purchase in Dan dong Chinese medicine hospital,Anhui Bozhou of arborvitae seed protein content in the highest.4 Platycladi seed’s total composition of the composition of the sedative,hypnotic and anti hypoxia effect.5 It is reasonable and feasible to make the preparation process of platycladi seed total constituents of the dripping pills,so that it is convenient for production and application.