| Background:Hyperlipidemia is an independent risk factor for atherosclerosis and coronary atherosclerotic heart disease;among them,low-density lipoprotein cholesterol is the basic factor leading to AS;moreover,elevated triglycerides can significantly increase hyperlipemia patients having cardiovascular disease,cerebrovascular disease,the risk of pancreatitis,diabetes and its complications.In addition,elevated triglycerides can also aggravate the pathological progression of albuminuria and the pathological development of diabetic nephropathy.In hyperlipidemia patients,drug therapy is mainly used in clinical treatment.Although Western medicine has a good performance in regulating lipids,especially lipid-lowering effects,once the drug is discontinued,the efficacy is reduced or even disappeared,which in turn leads to a rebound in blood lipids.The long-term application of lipid-lowering drugs,such as atorvastatin,simvastatin,etc.,there will be such as gastric pain,gastric ulcer and other adverse reactions.Therefore,it is extremely urgent and necessary to find a safe,effective and long-lasting treatment method to regulate blood lipids and keep them in a relatively stable state.In particular,exploring a scientific and rational clinical treatment plan is extremely urgent and necessary.Purpose:In this experiment,a high-fat diet was used to establish a rat model of hyperlipidemia.From the aspects of apolipoprotein C3 and lipoprotein lipase,lipid-lowering effects of intervention of blood stasis and diarrhea on hyperlipidemic rats were evaluated,and discuss its mechanism of action of hyperlipidemia intervention.Method:1.Experimental groups:32 male SD rats were randomly divided into 4 groups:normal group,model group,blood pricking group and positive drug group according to the random number table.Each group includ 8 rats.The rats in each group were fed for one week before the experiment.2.Modeling methods:In this study,we established the animal model of hyperlipidemia by using the most research had used.High-fat diet contains 82.8%basic feed,1%cholesterol,10%lard,0.2%propylthiouracil,5%egg yolk powder and 1%sodium cholate.28 days in a row.3.Intervention methods:(1)Normal group:Conventional feeding for 8 weeks without any treatment,starting from the 5th week,deionized water is given to the stomach,grabbing and pressing only,2 times per week.(2)Model group:High-fat diets were fed for 8 weeks.From the 5th week,deionized water was given to the stomach and only grabbed and pressed,twice a week.(3)Blood pricking group:High-fat diet was fed for 8 weeks.From the 5th week,deionized water was given to the stomach,Fenglong,Zusanli blood stasis,blood loss was 0.3ml-0.5ml,2 times per week.(4)Positive drug group:High-fat diets were fed for 8 weeks,starting from the 5th week and gavage at 1.8 mg/(kg.d)Lipitor twice a week.Index detection and methods:Enzyme-linked immunosorbent assay was used to detect serum lipids,ApoC3 content,and LPL activity in each group of rats.Result:1.Changes of four lipids in rats in each group:At the end of the experiment,the levels of TC and LDL in the model group were significantly higher than those in the normal group(P<0.05).Compared with the model group,the TC and LDL levels in the blood pricking group and positive drug group were lower than those in the model group,and the difference was statistically significant(P<0.05).There was no significant difference in the content of four lipids between the blood pricking group and the positive drug group(P>0.05).2.Changes of ApoC3 Content and LPL Activity in Rats:(1)Changes in ApoC3 content:Compared with the normal control group,the ApoC3 content in the model control group was significantly increased(P<0.05);compared with the model group,the ApoC3 content in the blood group and the positive drug group was significant.The difference was statistically significant(P<0.05).There was no significant difference in the content of ApoC3 between the pricking blood group and the positive drug group(P>0.05).(2)Changes in LPL activity:Compared with the normal group,the LPL activity in the model group was not significantly different(P>0.05);compared with the model group,the LPL activity in the blood group was not significantly different from that in the positive drug group(P>0.05);LPL activity was not significantly different between the pricking blood group and the positive drug group(P>0.05);there was no significant difference among the groups.Conclusion:1.Rats "Fenglong Point" and "Zusanli Point" pricked blood,can effectively reduce serum TC,TG,LDL-C content,increase HDL-C content,stabilize blood lipid levels.2.Rats "Fenglong Point" and "Zusanli Point" pricked blood to reduce blood serum ApoC3 content without affecting LPL activity. |
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