The Research On The Processing Technology Of Honeysuckle And Mountain Silver Flower And The Application Of One Test And Multi-evaluation Method In Honeysuckle

Flos Lonicerae Japonicae is the dry flower bud of Lonicera japonica Thunb. Flos Lonicerae is the dry flower bud of L. hypoglauca Miq.?L. confusa DC. and L. dasystyla Rehd. Author systematical to study and research the key point technique of harvest and preliminary processing Flos Lonicerae Japonicae and Flos Lonicerae;new method (quantitative analysis of multi-components by single marker)for quality evaluation in Flos Lonicerae Japonicae.1. The preliminary processing and character of finished product for flos lonicerae japonicae and flos loniceraeAccording to compare the influence of preliminary processing and character of finished product for flos lonicerae japonicae and flos lonicerae, optimization reasonable preliminary processing, for definite preliminary processing Flos Lonicerae Japonicae and Flos Lonicerae to establish foundation.2. The key point technique of harvest and preliminary processing flos lonicerae japonicae and flos loniceraeAccording to the analyses of the HPLC fingerprint profiles of flos lonicerae japonicae bud obtained by various dryness techniques(6 species 24 batch different dryness technique of flos lonicerae japonicae in Shandong an 5 species 20 batch different dryness technique of flos lonicerae in Henan), discover flos lonicerae japonicae acquired through the same place of production, the same species, the same time of harvest, but various dryness techniques, component has correlation and difference. Among to contain similar chemical constituent, but owing to various dryness techniques the relative amount of component has obviously difference, therefore to utilize flos lonicerae japonicae,we must pay attention to various dryness techniques affect its quality.According to the analyses of the software SPSS13.0, to divide sample for two species, deactivation of enzymes stoving for one, sulphur fume then open-air drying for another, therefore the HPLC fingerprint profiles can to classify the various dryness techniques.3. Quantitative analysis of multi-components by single marker--new method for quality evaluation in Lonicera japonicaTo evaluate comprehensively the quality of herbal medicines is a challenge because of their diversity of chemical ingredients. Current quality control patterns are limited to industrial application, for most of the natural chemical reference substances are unavailable commercially. Herein, a case study was conducted to determine the UV relative correction factors (fk/s) of two components by HPLC-DAD. And according to their fk/s at 365 nm, we developed a method for simultaneously determined these two components by using a single standard in Lonicera japonica.The contents of rutin in 14 samples of Lonicera japonica were authentically determined by the external standard method, and the contents of the chlorogenic acid and luteoloside were calculated according to their RCFuv. Comparison with accurate values obtained from classical method proved that our proposed method is a fast, convenient, and accurate method to determine multi-components especially when some authentic standards were unavailable. It can be used in quality control of Lonicera japonica.