Research For Symbiotic Bacteria Community Structure In Gut Of Termite And Locust

As fossil fuels become depleted,the shortage of the energy issue has become a common problem facing humanity,the new,clean,renewable energy development related to sustainable economic development and even survival of humanity.Research and utilization of various types of termites as the representative of natural and efficient biotransformation system(microbial flora),opens up a possible new way to overcome biomass efficient and economical conversion of international problems.This article has researched on termites(Tsaitermes ampliceps and Mironasutitermes shangchengensis),and five kinds of locusts,such as Locusta migratoria manilensis and Atractomorpha sinensis,their gut symbiotic bacteria of the community structure,and ultimately to clarify the mechanism of their gut lignocellulose degradationin-formation.1.We built 16S rDNA libraries of symbiont in gut of Tsaitermes ampliceps,using DGGE to screen and sequence the clone libraries.Sequence analysis showed that,16S rDNA gene gained from PCR amplification represented the 15 phylogenetic types of the termite gut,most of them were Spirochaetes,Clostridia and flagellate endosymbiont Pyrsonympha vertens,some of them belonged to Alpha proteobacteria and Delta proteobacteria,there was still a small amount of Bacillus.In which the dominant group is spirochete,abundance reached 28.07%;followed by were Clostridia and flagellate endosymbiont Pyrsonympha vertens,respectively,abundance 24.34%,and 23.28%;Bacillus was the least,only 2.12%.2.Through PCR-DGGE and tapping recycling sequencing analysis,we got 16 kinds of symbiotic bacteria phylogenetic type in gut of Mironasutitermes shangchengensis,mostly belonging to the beta,? and ?-Proteobacteria,the relative abundance reached 23.61%,17.16%and 12.63%;for Clostridium Clostridia and Fibrobacteres,relative abundance,respectively 9.94 and 9.62%;part belongs to the Spirochaetes and the bacteroidetes,relative abundance of 7.68%,7.37%and6.24%;a small number of Actinobacteria,the relative abundance was only 5.73%.3.We used PCR-DGGE to study five kinds of locusts(Atractomorpha sinensis.Acrida cinerea,Locusta migratoria manilensis,Gastrimargus marmoratus and Jerusalem cricket)intestinal commensal bacteria diversity,and finally found that Jerusalem cricket had the largest amount of intestinal commensal bacteria.DGGE profiles showed 20 bands.Followed by Locusta migratoria manilensis and Atractomorpha sinensis,respectively,had 13 and 14 bands;Acrida cinerea have 11 bands;Gastrimargus marmoratus Thunberg at least five.DGGE bands tapping recycled sequencing analysis show that a total of seven kinds of Locusta migratoria manilensis gut symbiotic bacteria phylogenetic type,in which Klebsiella was the most dominant groups,the relative abundance of 19.74%;followed by Bacillus cereus and xylose aureus 17.17%and 14.64%;again for the Bacillus and Arthrobacter is a relative abundance of 13.91%and 12.03%respectively;Pseudomonas aeruginosa and Enterobacter cloacae is a relatively small relative abundance of 11.61%and 10.90%respectively.A total of five kinds of phylogenetic type of symbiotic bacteria in the gut of Atractomorpha sinensis,where a-proteobacteria as the most dominant group,the relative abundance of 29.04%,followed by the actinomycetes and Bacillus,the relative abundance 19.72%and 19.95%;the spirochete and Streptococcus less,the relative abundance of 17.81%and 13.48%respectively.