The Construction Of The STM Mutant Library Of Symbiotic Bacteria Asaia Sp.in Anopheles And Screening Symbiotic Factors Related To Asaia Sp.

Malaria is a mosquito-borne human infectious disease caused by Plasmodium parasite.Together with AIDS and tuberculosis,malaria is among the deadliest infectious diseases.About 3.2 billion people are at risk of being infected with malaria and developing disease.One of the major objectives of malaria control programs is to interfere with parasite transmission inanopheles vectors.The emergenceand rapid spread of insecticide resistance mosquito and of drug-resistant Plasmodium parasites combined with the lack of effective vaccine severely undermine current intervention efforts,new tools to fight malaria are urgently needed.Mosquitomidgut contains a various bacteria,some of which were reported to be able to interfere with Plasmodium development in the midgut.More recently a great deal of attention has beendevoted to the development of another approach,basedon the manipulation of mosquito symbiotic bacteria,to block malaria transmission in the vector.In this study,using the signature-tagged mutagenesis method,we constructed the high efficient genetic transformation system of the genus Asaia sp.,a dominant symbiotic bacteria of malarial mosquitos,with the horizontal and vertical transmission ability not only has an important influence on the development of anopheles,also plays a important role in blocking malaria transmission.Twenty-three transposon vectors with different tags(S17-1? pir pbam3-gfp-tagn)have been constructed,two potential symbiotic factors was screened.The large number of mutants(about 44 hundreds)provide rich resources for thesystemic screening of symbiotic factors relate to Asaia sp.