The Toxicological Effect And Change In Intestinal Microorganism On The Carassius Auratus Gibelio After The Treatment Of Sulfadiazine

Antibiotics pollution is environmental issues of concern in recent years,especially the antibiotics of water environment,not only can they produce certain effects to aquatic organisms,but also will enter the human body through the food chain transmission,which may cause harm to human body.In this article,studies were made respectively on the bioaccumulation,clear,histopathological dynamic changes of sulfadiazine in the tissues of Carassius auratus gibelioallo and change in intestinal microorganism of the Carassius auratus gibelio through after exposing the fish to different concentrations of sulfadiazineby a semi-statictest method,to provide some theoretical guidances for the rational use of sulfonamides drugs and the diagnosis of diseases in Carassius auratus gibelio.The main conclusions were summarized as follows:(1)Carassius auratus gibelio were treated with sulfadiazine and then the bioaccumulation and elimination of sulfadiazine in tissues were analyzed by determining the content of sulfadiazine in liver,kidney,gill,intestinal and muscle.The results indicated that the ability of each tissue to bioaccumulation of sulfadiazine is kidney > liver > gill >intestine > muscle,which showed that kidney and liver were two main organs of sulfadiazine bioaccumulation.Water purification stage,the change trends of the contents of sulfadiazine with different concentrationswere relatively consistent,which wereall in rapid decline,showingthat the water has a better removal effect on the concentration in the tissue of sulfadiazine in Carassius auratus gibelio.(2)The degree of damage of sulfadiazine to the tissues(liver,kidney,gill,intestine,muscle)of Carassius auratus gibelio was studied by pathology.The results suggested that different pathological changes occurred on the liver,kidney,gill and muscle of Carassius auratus gibelio under different concentrations of sulfadiazine stress,which included the congestion,vacuolation,karyopyknosis,necrosis of liver cell,the vacuolar degeneration of epithelial cells,necrocytosis in glomerulus and congestion in renal interstitium,the edema of epithelial cell in gill lamellae,hyperplasia and disappearance of gill lamellae,the exfoliation of microvilli,thicker submucosa and vacuoles degeneration and necrosis of muscularis,the widened gap,disorder and fragmentation of myofiber.Among all the tissues,liver,gill and kidneypresented more serious pathological lesions than other two tissues and damage of tissues was inhibited obviously in a time and dose dependant manner.(3)By detecting gut microbes ofCarassius auratus gibelio treated before and after Sulfadiazine stress,The effect of sulfadiazine on Carassius auratus gibelio intestinal microbes was analyzed.The results showed that Firmicutes,ProteobacteriaandBacteroidateswere the predominant phyla in Carassius auratus gibelio intestine.With different concentrations of Sulfadiazine treated,not only thecontants of Desulfovlbrlonaceae,Paeudomonas and Hellcobacter in gut decreased observably,but also the numbers of Bifidobacterium,Bacillus and Lactobacillus.Sulfadiazine led to the microbial diversity decreased significantly and certain pathogenic bacteria could be controlled by Sulfadiazine but it also had an nagtive impact on some probiotics.