The Study Of DNA Damage And Purine Metabolism Based On Single Walled Carbon Nanotube Electrodes

As a favorite of nanomaterials,carbon nanotubes(CNTs)raise concern in many fields due to their large specific surface area,strong adsorptive ability,excellent conductivity and good biocompatibility.In this dissertation,two methods were used to modify CNTs,and two electrochemical sensors were constructed,respectively.The main contents of the study are as follows: 1.In this work,a facile strategy was proposed to prepare porous single-walled carbon nanotubes(PSWNTs)via a green carbon thermal reduction.The morphology,structure and electrochemical properties of PSWNTs were investigated by transmission electron microscopy(TEM),X-ray diffraction(XRD),nitrogen adsorption-desorption(BET),cyclic voltammetry(CV)and electrochemical impedance spectroscopy(EIS).TEM and BET investigations indicated the average pore size of PSWNTs is about 3 nm.The electrochemical experiments demonstrated that PSWNTs possess relatively large surface area,strong adsorptive ability and excellent electrical conductivity.With the help of the unique porous structure and inherent properties of carbon nanotubes,the PSWNTs/GCE showed outstanding electrochemical performance for 8-Hydroxy-2?-deoxyguanosine(8-OHd G)with a detection limit of 1.0 nM.Moreover,the influence factors of DNA damage were also studied in detail,and relevant kinetics parameters are obtained.2.From the first chapter,we found that single wall carbon nanotubes(SWNTs)have excellent conductivity and good biocompatibility.Thus,in this chapter,SWNTs were covalently functionalized with glutamic acid(PGA).PGA-SWNTs were characterized by different methods,and showed specific surface area and desirable electrical conductivity.Then a facile electrochemical sensor was fabricated based on PGA-SWNTs composites.The sensor was employed for study of the electrochemical properties of xanthine and the kinetic process of purine metabolism.Meanwhile,the inhibition of febuxostat for the formation of uric acid has been researched by electrochemistry.