The Resistance Analysis And Molecular Typing Of Staphylococcus Aureus From Poultry In Anhui Area

Staphylococcus aureus is a representative strain of the genus Staphylococcus and is currently recognized as one of the major pathogenic microorganisms that cause human infectious diseases.Antibiotics are commonly used in clinical treatment.However,with the abuse of antibiotics,more and more strains of Staphylococcus aureus have emerged in humans and animals.More surprisingly,with the increasing drug resistance,multidrug-resistance has become more and more common,which has had a tremendous impact on the use of antibiotics in the prevention and treatment of S.aureus infections.Therefore,studying the resistance and epidemiological characteristics of poultry-derived Staphylococcus aureus in Anhui area not only has guiding significance for clinical rational drug use,but also further reduces and controls the spread of Staphylococcus aureus by understanding its epidemic trend and regularity.In this study,liver tissues from diseased poultry were collected from some parts of Anhui as samples.Gram stain,microscopic examination,selection of culture media,pathogenicity verification test,and PCR identification were performed to isolate and identify Staphylococcus aureus.Then multidrug-resistant Staphylococcus aureus screened by drug sensitivity test.The results showed that 103 strains of multi-resistant Staphylococcus aureus were screened out.Among them,?-lactams account for up to 19%,followed by aminoglycosides 17%,rifampicin 15%,macrolides 14%,fosfomycin 11%,thiamins 10%,amido alcohols 9 %,tetracyclines are at least 5%.The strains were resistant to four(21/103),five(43/103)and six(22/103)drug resistance cases.The five-drug resistance was the highest(41.7%)and was mainly resistant to ?-lactams(31/43),macrolides(27/43),and aminoglycosides(29/43).Followed by six resistance,mainly resistant to ?-lactams(19/22),aminoglycosides(19/22)and rifampicin(19/22);quadruple resistance,mainly resistant to ?-lactams(17/21),Sulfonamides(17/21)and Aminoglycosides(15/21).The antimicrobial resistance of 103 strains of multi-resistant Staphylococcus aureus was detected.The detection rates were mecA,aac(6')/aph(2”),ermC,ermA and femA,and the drug resistance phenotypes were high to low,basically the same.It is shown that the resistance of Staphylococcus aureus is highly diversified,and the carrying rates of aminoglycosides,?-lactams,and macrolide antibiotics are relatively high.103 strains of multi-resistant Staphylococcus aureus were subjected to ERIC-PCR typing.In the same region of Anhui area,isolates of different animal origins or isolates of the same origin in different regions have the same genotype,and there are also different genotypes;and the genotypes of isolates in different regions also belong to the same genotype.At the same time,strains with large genetic distances and relatively different genetic distances also appeared.In summary,this study analyzed the resistance of poultry-derived Staphylococcus aureus in Anhui area and the epidemiological characteristics of the study,identified the drug-resistant conditions of the isolates of Staphylococcus aureus in Anhui area,and initially understood the prevalence of Staphylococcus aureus.The epidemic status of staphylococcus provides a theoretical basis for guiding clinical rational drug use in Anhui area,and lays the foundation for further exploration of the epidemic trend of the bacteria.