Research On Batch Nuclear Transfer Based On Micro Groove

Nuclear transplantation is the key technology in the fields of cloning,gene therapy and organ transplantation.It is important to improve the efficiency of nuclear transplantation to promote the development of these fields.In this paper,the demand for batch nuclear transfer is improved in three aspects: experimental device,key technology and operation process.A batch nuclear transfer method based on micro groove is proposed to achieve high efficient batch nuclear transfer.First,a batch cell transport method based on micro groove is proposed.A micro groove for placing cells is designed to limit the cell's movement range,so that there is no need to transform the field of vision in operation and reduce cell search.In order to adapt to the new operation assistant tools,this paper optimized the experimental process of cell transport.In this paper,the force of cell at the bottom,suction and release of the groove is analyzed.The minimum pressure of is obtained by Bernoulli equation.The minimum pressure of release is obtained by the conservation of moment.On this basis,taking cell toggle as an example,the superiority of the batch cell transport method proposed in this paper is verified.Experimental results show that the efficiency of this method is 20% higher than that of traditional methods.Secondly,aiming at the demand of batch injection,this paper presents a method of microinjection control based on balance pressure to achieve high precision injection control.The optical flow method is the real-time feedback of the interface position and velocity.The model between the pressure and gas-liquid interface position is established.Based on the model,the feedback linearization control method is used to control the system.The gas liquid interface position control experiment shows the effectiveness of the method,improves efficiency and reduces overshoot.Furthermore,the quantitative injection of NBCD in Drosophila proves that the method has good biological application effect.Third,batch nuclear transfer experiments were carried out in this paper.Cell transport by micro groove proposed in this paper is used to reduce the transformation of the object lens and the field of vision,the function separation of the enucleation and the injection is carried out to improve the efficiency.The cells are regarded as no relative motion with the culture medium,and the control method of the position of the gas and liquid boundary is used to control the cell position.The results of nuclear transplantation experiments show that the efficiency of the proposed method is increased by 25% compared with the previous method.